Expression patterns of the native Shrunken-2 promoter in Sorghum bicolor visualised through use of the GFP reporter gene

Key message The AGPase large subunit (shrunken-2) promoter was demonstrated to be active in the placentochalaza and endosperm of developing grain as well as the root tips in transgenic sorghum. The temporal and spatial expression patterns of the Sorghum bicolor Shrunken - 2 ( Sh2 ) promoter were eva...

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Bibliographic Details
Published in:Plant cell reports 2017-11, Vol.36 (11), p.1689-1700
Main Authors: Lamont, Kyle C., Mudge, Stephen R., Liu, Guoquan, Godwin, Ian D.
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cell Biology
Confocal microscopy
Endosperm
Epidermis
Fluorescence
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Glucose-1-Phosphate Adenylyltransferase - genetics
Glucose-1-Phosphate Adenylyltransferase - metabolism
Grain
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Life Sciences
Microscopy
Original Article
Plant Biochemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Sciences
Plant tissues
Plants, Genetically Modified - genetics
Plants, Genetically Modified - metabolism
Pollen
Promoter Regions, Genetic - genetics
Reporter gene
Scanning microscopy
Sorghum
Sorghum - genetics
Sorghum - metabolism
Sorghum bicolor
Starch
Tips
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Summary:Key message The AGPase large subunit (shrunken-2) promoter was demonstrated to be active in the placentochalaza and endosperm of developing grain as well as the root tips in transgenic sorghum. The temporal and spatial expression patterns of the Sorghum bicolor Shrunken - 2 ( Sh2 ) promoter were evaluated using the green fluorescence protein reporter gene ( gfp ) in transgenic sorghum, within the context of upregulating starch biosynthesis in the developing grain. GFP fluorescence was analysed throughout development in various tissue types using confocal laser scanning microscopy techniques. Sh2 promoter activity was first detected in the placentochalaza region of the developing caryopsis and apoplasm adjacent to the nucellar epidermis at 7 days post anthesis (dpa) where fluorescence remained relatively constant until 17 dpa. Fluorescence in this region weakened by 20 dpa and disappeared by 25 dpa. Expression was also detected in the developing endosperm, but not until 12 dpa, continuing until 25 dpa. Whilst the endosperm expression was expected, the fluorescence detected in the placentochalaza was completely unexpected. Although transcript presence does not mean the resulting biochemistry is also present, these preliminary findings may suggest alternate spatial activity of ADP-glucose pyrophosphorylase prior to uptake by the developing grain. Sh2 promoter activity was also unexpectedly detected in the root tips at all developmental time points. Sh2 promoter activity was not detected in any reproductive floral tissue (both pre and post anthesis) or in pollen. Similarly, no expression was detected in leaf tissue at any stage.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-017-2182-4