Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development

Background: Tooth development is known to be mediated by the cross‐talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19‐ to 25‐nt noncoding small single‐stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be imp...

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Published in:Developmental dynamics 2012-09, Vol.241 (9), p.1465-1472
Main Authors: Oommen, Shelly, Otsuka-Tanaka, Yoko, Imam, Najam, Kawasaki, Maiko, Kawasaki, Katsushige, Jalani-Ghazani, Farnoosh, Anderegg, Angela, Awatramani, Rajeshwar, Hindges, Robert, Sharpe, Paul T., Ohazama, Atsushi
Format: Article
Language:English
Subjects:
Animals
DEAD-box RNA Helicases - genetics
DEAD-box RNA Helicases - metabolism
Dicer
Embryo, Mammalian
epithelial-mesenchymal interaction
Epithelium - embryology
Epithelium - metabolism
Gene Expression Regulation, Developmental - physiology
Hedgehog Proteins - genetics
Hedgehog Proteins - metabolism
Integrases - genetics
Integrases - metabolism
Mesoderm - embryology
Mesoderm - metabolism
Mice
Mice, Transgenic
Microarray Analysis
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
MicroRNAs - physiology
Odontogenesis - genetics
Ribonuclease III - genetics
Ribonuclease III - metabolism
ShhCre
Tooth - cytology
Tooth - embryology
Tooth - metabolism
tooth development
Transcriptome
Wnt1 Protein - genetics
Wnt1 Protein - metabolism
Wnt1Cre
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Summary:Background: Tooth development is known to be mediated by the cross‐talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19‐ to 25‐nt noncoding small single‐stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine‐tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal (Wnt1Cre/Dicerfl/fl) or epithelial (ShhCre/Dicerfl/fl) conditional deletion of Dicer, which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicerfl/fl, we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicerfl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicerfl/fl mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465–1472, 2012. © 2012 Wiley Periodicals, Inc. Key findings: Extra incisor tooth formation was found in ShhCre/Dicerfl/fl mice. Wnt1Cre/Dicerfl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Microarray an in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.23828