Combined photodynamic and antibiotic therapy for skin disorder via lipase-sensitive liposomes with enhanced antimicrobial performance

A lipase-sensitive singlet oxygen-producible and erythromycin-loaded liposome (LSSPL) was developed for combination antibacterial therapy for skin disorder. The LSSPL was synthesized by coating pullulan-pheophorbide a (PU-Pheo A) conjugates onto erythromycin-loaded liposomes composed of 1,2-dipalmit...

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Bibliographic Details
Published in:Biomaterials 2017-10, Vol.141, p.243-250
Main Authors: Jeong, Songhee, Lee, Jonghwan, Im, Byeong Nam, Park, Hyung, Na, Kun
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - therapeutic use
Antimicrobial therapy
Candida - drug effects
Candida - enzymology
Delayed-Action Preparations - chemistry
Delayed-Action Preparations - metabolism
Erythromycin - administration & dosage
Erythromycin - therapeutic use
Gram-Positive Bacterial Infections - drug therapy
Gram-Positive Bacterial Infections - microbiology
Lipase - metabolism
Lipase-sensitivity
Liposome
Liposomes - chemistry
Liposomes - metabolism
Male
Mice, Inbred BALB C
Mice, Nude
Propionibacterium acnes
Propionibacterium acnes - drug effects
Propionibacterium acnes - enzymology
Skin - drug effects
Skin - microbiology
Skin Diseases, Bacterial - drug therapy
Skin Diseases, Bacterial - microbiology
Skin disorder
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Description
Summary:A lipase-sensitive singlet oxygen-producible and erythromycin-loaded liposome (LSSPL) was developed for combination antibacterial therapy for skin disorder. The LSSPL was synthesized by coating pullulan-pheophorbide a (PU-Pheo A) conjugates onto erythromycin-loaded liposomes composed of 1,2-dipalmitoyl-sn-phosphatidylcholine (DPPC) and cholesterol. Lipase activity was chosen as the environmental-stimulus for the controlled release of erythromycin and Pheo A from LSSPL because skin inflammation-inducing Propionibacterium acnes (P. acnes) secrete extracellular lipases. The presence of P. acnes lipases disrupted LSSPLs by selective cleavage of their ester linkages, liberating erythromycin and Pheo A. Along with the antibacterial effect of erythromycin, additional laser irradiation onto Pheo A further achieved the inhibition of P. acnes growth and treatment of P. acnes-infected inflammation in nude mice back skin. Therefore, antimicrobial therapy, using a stimulus-responsiveness moiety, presents a feasible way to treat bacteria-induced skin disorders.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2017.07.009