Transcriptional Coactivation of Nuclear Factor-κB-dependent Gene Expression by p300 Is Regulated by Poly(ADP)-ribose Polymerase-1

Nuclear factor κB (NF-κB) plays an important role in the transcriptional regulation of genes involved in inflammation and cell survival. In this study, we demonstrated that NF-κB-dependent gene expression was inhibited by E1A in poly(ADP)-ribose polymerase-1 knock out (PARP-1 (–/–)) cells complement...

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Bibliographic Details
Published in:The Journal of biological chemistry 2003-11, Vol.278 (46), p.45145-45153
Main Authors: Hassa, Paul O., Buerki, Christine, Lombardi, Cornelia, Imhof, Ralph, Hottiger, Michael O.
Format: Article
Language:English
Subjects:
AE1A-associated p300 protein
E1A-associated p300 protein
p50 protein
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Summary:Nuclear factor κB (NF-κB) plays an important role in the transcriptional regulation of genes involved in inflammation and cell survival. In this study, we demonstrated that NF-κB-dependent gene expression was inhibited by E1A in poly(ADP)-ribose polymerase-1 knock out (PARP-1 (–/–)) cells complemented with wild type PARP-1 after tumor necrosis factor α (TNFα) or lipopolysaccharide (LPS) treatment. PARP-1 and p300 synergistically coactivated NF-κB-dependent gene expression in response to TNFα and LPS. Furthermore, PARP-1 interacted directly with p300 and enhanced the interaction of NF-κB1/p50 to p300. The C terminus, harboring the catalytic domain of PARP-1 but not its enzymatic activity, was required for complete transcriptional coactivation of NF-κB by p300 in response to TNFα and LPS. Together, these results indicate that PARP-1 acts synergistically with p300 and plays an essential regulatory role in NF-κB-dependent gene expression.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M307957200