Dominance‐function relationships in the amphibian skin microbiome

Summary Some amphibian skin bacteria inhibit growth of a fungal amphibian pathogen, Batrachochytrium dendrobatidis (Bd), but it is unclear how dominant these anti‐Bd bacteria are in skin communities. Using in vitro co‐culture challenge assays, we quantified Bd inhibition by bacterial isolates collec...

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Bibliographic Details
Published in:Environmental microbiology 2017-08, Vol.19 (8), p.3387-3397
Main Authors: Walke, Jenifer B., Becker, Matthew H., Hughey, Myra C., Swartwout, Meredith C., Jensen, Roderick V., Belden, Lisa K.
Format: Article
Language:English
Subjects:
Abundance
Amphibia
Amphibians
Animals
Antibiosis - physiology
Anura - microbiology
Aquatic reptiles
Bacteria
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Batrachochytrium dendrobatidis
Chytridiomycota - growth & development
Disease resistance
Frogs
Fungi
In vitro methods and tests
Inhibition
Microbiomes
Microbiota
Microorganisms
Pathogens
Populations
Relative abundance
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sequences
Skin
Skin - microbiology
Spring
Toads
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Summary:Summary Some amphibian skin bacteria inhibit growth of a fungal amphibian pathogen, Batrachochytrium dendrobatidis (Bd), but it is unclear how dominant these anti‐Bd bacteria are in skin communities. Using in vitro co‐culture challenge assays, we quantified Bd inhibition by bacterial isolates collected from the skin of four amphibian species: bullfrogs, Eastern newts, spring peepers and American toads. The 16S rRNA sequences for each isolate were matched to culture‐independent amplicon sequences from the same individuals to assess inhibitory function versus relative abundance. Dominant bacteria had higher Bd inhibition than rare bacteria in bullfrog and newt populations, in which Bd was prevalent (> 25%). Dominant and rare bacteria did not differ in Bd inhibition in spring peeper and toad populations, in which Bd was absent or at low prevalence (
ISSN:1462-2912
1462-2920
DOI:10.1111/1462-2920.13850