IN VITRO ORGANOGENESIS OF PELECYPHORA ASELLIFORMIS ERHENBERG (CACTACEAE)

In vitro propagation of Pelecyphora aselliformis, a Mexican cactus which is considered rare and is highly valued in the commercial market, was initiated using seeds as explants. The longitudinal explants from seedlings germinated in vitro were cultivated on Murashige and Skoog medium containing 8.8...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2003-09, Vol.39 (5), p.480-484
Main Authors: SANTOS-DÍAZ, M. A R. I A D. E. L S. OCORRO, MÉNDEZ-ONTIVEROS, R. I C. A R. D. O., ARREDONDO-GÓMEZ, A. L B. E R. T. O., SANTOS-DÍAZ, M. A R. I A D. E L. O. URDES
Format: Article
Language:English
Subjects:
benzyladenine
Biological and medical sciences
Cactaceae
cacti and succulents
Cactus
Callus formation
Endangered species
Epicotyls
explants
Fundamental and applied biological sciences. Psychology
hyperhydricity
in vitro culture
kinetin
Micropropagation
organogenesis
Pelecyphora
Pelecyphora aselliformis
Plant growth
Plant physiology and development
Plant propagation
Plants
Protocols/Methods
Rooting
seedlings
Seeds
shoots
Tissue cultures, protoplasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In vitro propagation of Pelecyphora aselliformis, a Mexican cactus which is considered rare and is highly valued in the commercial market, was initiated using seeds as explants. The longitudinal explants from seedlings germinated in vitro were cultivated on Murashige and Skoog medium containing 8.8 μM benzyladenine (BA) or 4.6 μM kinetin at pH 7.0. After 120 d, each explant gave rise to five shoots and this number of shoots increased 20–25% after subculture. The hyperhydricity was similar in both media, but callus formation was lower on the medium with BA. The shoot development, in terms of epicotyl length, and fresh and dry weight after 6 wk, was also recorded. The epicotyl length was similar on shoot-forming media but the quality of shoots was better on media containing BA. In about 1 yr, 500-600 well-defined shoots were obtained. The rooting of shoots was very slow and a vigorous radical system was observed after 1 yr of culture.
ISSN:1054-5476
1475-2689
DOI:10.1079/IVP2003456