LEI0258 microsatellite variability and its association with humoral and cell mediated immune responses in broiler chickens

•There is no information about MHC genotypes and disease resistance in Ross 308 broiler chicken.•A significant association was observed between LEI0258 and Cell-mediated immune response.•A significant association was observed between LEI0258 and immune response to Infectious bursal disease (IBD).•A...

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Bibliographic Details
Published in:Molecular immunology 2017-10, Vol.90, p.22-26
Main Authors: Esmailnejad, Atefeh, Nikbakht Brujeni, Gholamreza, Badavam, Maryam
Format: Article
Language:English
Subjects:
Alleles
Animals
Birnaviridae Infections - immunology
Birnaviridae Infections - veterinary
Birnaviridae Infections - virology
Broiler chicken
Cell mediated immune responses
Cell Proliferation
Chickens - genetics
Chickens - immunology
Genetic Markers - genetics
Humoral immune responses
Immunity, Cellular - genetics
Immunity, Humoral - genetics
Influenza in Birds - immunology
Influenza in Birds - virology
Influenza Vaccines - immunology
LEI0258
Major Histocompatibility Complex - genetics
Major Histocompatibility Complex - immunology
MHC
Microsatellite Repeats - genetics
Newcastle Disease - immunology
Newcastle Disease - virology
Polymorphism, Genetic - genetics
Viral Vaccines - immunology
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Summary:•There is no information about MHC genotypes and disease resistance in Ross 308 broiler chicken.•A significant association was observed between LEI0258 and Cell-mediated immune response.•A significant association was observed between LEI0258 and immune response to Infectious bursal disease (IBD).•A significant association was observed between LEI0258 and immune response to Newcastle disease (ND). Major histocompatibility complex (MHC) has a profound influence on disease resistance or susceptibility, productivity and important economic traits in chicken. Association of the MHC with a wide range of immune responses makes it a valuable predictive factor for the disease pathogenesis and outcome. The tandem repeat LEI0258 is a genetic marker which is located within the B locus of chicken MHC and strongly associated with serologically defined haplotypes. LEI0258 microsatellite marker was applied to investigate the MHC polymorphism in Ross 308 broiler chicken (N=104). Association of LEI0258 alleles with humoral and cell mediated immune responses to Newcastle disease (ND), Infectious bursal disease (IBD) and Avian influenza (AI) vaccines were also examined. LEI0258 polymorphism was determined by PCR-based fragment analysis, and association of LEI0258 alleles with immune responses were evaluated using multivariate regression analysis and GLM procedures. A total of seven alleles ranging from 195 to 448bp were found, including two novel alleles (263 and 362bp) that were unique in Ross 308 broiler population. Association study revealed a significant influence of MHC alleles on humoral and cellular immune responses in Ross population (P
ISSN:0161-5890
1872-9142
DOI:10.1016/j.molimm.2017.06.027