Molecular mechanisms and relationship of M2-polarized macrophages with early response in multiple myeloma

To investigate the relationship between M2-polarized macrophages and early response in multiple myeloma and its molecular mechanism. Two hundred and forty bone marrow biopsy tissue were collected and M2-polarized macrophages were stained by anti-CD163 monoclonal antibody. In vitro M2-polarized macro...

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Bibliographic Details
Published in:Zhōnghuá xuèyèxué zázhì 2017-06, Vol.38 (6), p.480-486
Main Authors: Chen, X Y, Sun, R X, Zhang, W Y, Liu, T, Zheng, Y H, Wu, Y
Format: Article
Language:Chinese
Subjects:
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Cell Line
Coculture Techniques
Cytokines
Humans
Macrophages
Multiple Myeloma
Receptors, Cell Surface
Vascular Endothelial Growth Factor Receptor-1
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Summary:To investigate the relationship between M2-polarized macrophages and early response in multiple myeloma and its molecular mechanism. Two hundred and forty bone marrow biopsy tissue were collected and M2-polarized macrophages were stained by anti-CD163 monoclonal antibody. In vitro M2-polarized macrophages were derived from human peripheral blood mononuclear cell or THP-1 cells and identified by flow cytometry. Two myeloma cell lines RPMI 8226 and U266 were co-cultured with M2 macrophages using a transwell system. We measured myeloma cells proliferation through CCK-8 method and the pro-inflammatory cytokines expression (TNF-α and IL-6) by ELISA. Real time PCR was applied to measure chemokines (CCL2 and CCL3) , chemokine receptors (CCR2, CCR5) , VEGF and their receptors. In addition, flow cytometry was used to analyze the apoptosis of myeloma cells induced by dexamethasone. ①Patients with high percentage of M2 macrophage involvement in bone marrow showed poorer response (23.9% versus 73.0%, (2)=60.31,
ISSN:0253-2727
DOI:10.3760/cma.j.issn.0253-2727.2017.06.004