Anti-Tuberculosis Bacteriophage D29 Delivery with a Vibrating Mesh Nebulizer, Jet Nebulizer, and Soft Mist Inhaler

Purpose To compare titer reduction and delivery rate of active anti-tuberculosis bacteriophage (phage) D29 with three inhalation devices. Methods Phage D29 lysate was amplified to a titer of 11.8 ± 0.3 log 10 (pfu/mL) and diluted 1:100 in isotonic saline. Filters captured the aerosolized saline D29...

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Bibliographic Details
Published in:Pharmaceutical research 2017-10, Vol.34 (10), p.2084-2096
Main Authors: Carrigy, Nicholas B., Chang, Rachel Y., Leung, Sharon S. Y., Harrison, Melissa, Petrova, Zaritza, Pope, Welkin H., Hatfull, Graham F., Britton, Warwick J., Chan, Hak-Kim, Sauvageau, Dominic, Finlay, Warren H., Vehring, Reinhard
Format: Article
Language:English
Subjects:
Administration, Inhalation
Aerosols
Aerosols - chemistry
Animals
Bacteriophages
Biochemistry
Biomedical and Life Sciences
Biomedical Engineering and Bioengineering
Biomedicine
Dilution
Drug Liberation
Drug Stability
Equipment and Supplies
Equipment Design - methods
Filters
Humans
Inhalation
Inhalers
Medical Law
Mist
Nebulizers and Vaporizers
Phage
Phage Therapy
Phages
Pharmacology/Toxicology
Pharmacy
Research Paper
Respiration
Respiratory agents
Saline
Self-administration
Tuberculosis
Tuberculosis - therapy
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Summary:Purpose To compare titer reduction and delivery rate of active anti-tuberculosis bacteriophage (phage) D29 with three inhalation devices. Methods Phage D29 lysate was amplified to a titer of 11.8 ± 0.3 log 10 (pfu/mL) and diluted 1:100 in isotonic saline. Filters captured the aerosolized saline D29 preparation emitted from three types of inhalation devices: 1) vibrating mesh nebulizer; 2) jet nebulizer; 3) soft mist inhaler. Full-plate plaque assays, performed in triplicate at multiple dilution levels with the surrogate host Mycobacterium smegmatis , were used to quantify phage titer . Results Respective titer reductions for the vibrating mesh nebulizer, jet nebulizer, and soft mist inhaler were 0.4 ± 0.1, 3.7 ± 0.1, and 0.6 ± 0.3 log 10 (pfu/mL). Active phage delivery rate was significantly greater ( p  
ISSN:0724-8741
1573-904X
DOI:10.1007/s11095-017-2213-4