Synthesis, biological evaluation and docking studies of trans‐stilbene methylthio derivatives as cytochromes P450 family 1 inhibitors

Cytochromes P450 family 1 (CYP1) are responsible for the metabolism of procarcinogens, for example polycyclic aromatic hydrocarbons and aromatic and heterocyclic amines. The inhibition of CYP1 activity is examined in terms of chemoprevention and cancer chemotherapy. We designed and synthesized a ser...

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Published in:Chemical biology & drug design 2017-12, Vol.90 (6), p.1226-1236
Main Authors: Wierzchowski, Marcin, Dutkiewicz, Zbigniew, Gielara‐Korzańska, Agnieszka, Korzański, Artur, Teubert, Anna, Teżyk, Artur, Stefański, Tomasz, Baer‐Dubowska, Wanda, Mikstacka, Renata
Format: Article
Language:English
Subjects:
Binding Sites
Cytochrome P-450 CYP1A1 - chemistry
Cytochrome P-450 CYP1A1 - genetics
Cytochrome P-450 CYP1A1 - metabolism
Cytochrome P-450 CYP1A2 - chemistry
Cytochrome P-450 CYP1A2 - genetics
Cytochrome P-450 CYP1A2 - metabolism
Cytochrome P-450 CYP1B1 - chemistry
Cytochrome P-450 CYP1B1 - genetics
Cytochrome P-450 CYP1B1 - metabolism
Cytochrome P450 Family 1 - chemistry
Cytochrome P450 Family 1 - genetics
Cytochrome P450 Family 1 - metabolism
cytochromes P450 family 1
Enzyme Inhibitors - chemical synthesis
Enzyme Inhibitors - metabolism
Humans
Isomerism
Magnetic Resonance Spectroscopy
Molecular Conformation
molecular docking
Molecular Docking Simulation
Protein Structure, Tertiary
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Stilbenes - chemistry
Stilbenes - metabolism
Thermodynamics
trans‐resveratrol analogues
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Summary:Cytochromes P450 family 1 (CYP1) are responsible for the metabolism of procarcinogens, for example polycyclic aromatic hydrocarbons and aromatic and heterocyclic amines. The inhibition of CYP1 activity is examined in terms of chemoprevention and cancer chemotherapy. We designed and synthesized a series of trans‐stilbene derivatives possessing a combination of methoxy and methylthio functional groups attached in different positions to the trans‐stilbene skeleton. We determined the effects of synthesized compounds on the activities of human recombinant CYP1A1, CYP1A2 and CYP1B1 and, to explain the variation of inhibitory potency of methoxystilbene derivatives and their methylthio analogues, we employed computational analysis. The compounds were docked to CYP1A1, CYP1A2 and CYP1B1 binding sites with the use of Accelrys Discovery Studio 4.0 by the CDOCKER procedure. For CYP1A2 and CYP1B1, values of scoring functions correlated well with inhibitory potency of stilbene derivatives. All compounds were relatively poor inhibitors of CYP1A2 that possess the most narrow and flat enzyme cavity among CYP1s. For the most active CYP1A1 inhibitor, 2‐methoxy‐4′‐methylthio‐trans‐stilbene, a high number of molecular interactions was observed, although the interaction energies were not distinctive. A series of trans‐stilbene derivatives possessing a combination of methoxy and methylthio functional groups were synthesized in order to evaluate the influence of the structure variations on the affinity of ligands to CYP1 cytochromes. We attempted to elucidate the diversified inhibitory activities of polymethoxy/methylthio trans‐stilbenes with the use of computational methods. Docking studies showed a good correlation between experimental data and predicted affinities for CYP1A2 and CYP1B1 giving insight into essential interactions of trans‐stilbenes in CYP1 binding sites.
ISSN:1747-0277
1747-0285
DOI:10.1111/cbdd.13042