Rational Engineering of a Dynamic, Entropy‐Driven DNA Nanomachine for Intracellular MicroRNA Imaging

We rationally engineered an elegant entropy‐driven DNA nanomachine with three‐dimensional track and applied it for intracellular miRNAs imaging. The proposed nanomachine is activated by target miRNA binding to drive a walking leg tethered to gold nanoparticle with a high density of DNA substrates. T...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2017-07, Vol.56 (31), p.9077-9081
Main Authors: Liang, Cheng‐Pin, Ma, Pei‐Qiang, Liu, Hui, Guo, Xinggang, Yin, Bin‐Cheng, Ye, Bang‐Ce
Format: Article
Language:English
Subjects:
Catalysis
catalytic reactions
Cell Line
Cells (biology)
Deoxyribonucleic acid
Design improvements
Dismantling
DNA
DNA - chemistry
DNA - metabolism
DNA nanomachines
Engineering
Entropy
Fluoresceins - chemistry
Fluorescence
Gold
Gold - chemistry
gold nanoparticles
Heat of reaction
HeLa Cells
Humans
Imaging
Intracellular
Kinetics
Leg
Metal Nanoparticles - chemistry
microRNA
MicroRNAs - chemistry
MicroRNAs - metabolism
Microscopy, Confocal
miRNA
Nanoparticles
Nucleic Acid Hybridization
Reaction kinetics
Ribonucleic acid
RNA
sensors
Substrates
Walking
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Summary:We rationally engineered an elegant entropy‐driven DNA nanomachine with three‐dimensional track and applied it for intracellular miRNAs imaging. The proposed nanomachine is activated by target miRNA binding to drive a walking leg tethered to gold nanoparticle with a high density of DNA substrates. The autonomous and progressive walk on the DNA track via the entropy‐driven catalytic reaction of intramolecular toehold‐mediated strand migration leads to continuous disassembly of DNA substrates, accompanied by the recovery of fluorescence signal due to the specific release of a dye‐labeled substrate from DNA track. Our nanomachine outperforms the conventional intermolecular reaction‐based gold nanoparticle design in the context of an improved sensitivity and kinetics, attributed to the enhanced local effective concentrations of working DNA components from the proximity‐induced intramolecular reaction. Moreover, the nanomachine was applied for miRNA imaging inside living cells. Walk on a DNA track: An elegant entropy‐driven DNA nanomachine with a three‐dimensional track was rationally engineered (see picture). The nanomachine was applied for microRNA imaging inside living cells.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201704147