Can γ-radiation modulate hemagglutinating and anticoagulant activities of PpyLL, a lectin from Phthirusa pyrifolia?

•Biological implications of proteins may be elucidated under radiation assays.•Lectin may be partial affected by low doses of gamma radiation.•Radiation modulates lectin anticoagulant activity.•A single binding site of PpyLL may be resistant to high radiation dose. Blood coagulation and platelet-dep...

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Bibliographic Details
Published in:International journal of biological macromolecules 2017-11, Vol.104 (Pt A), p.125-136
Main Authors: Costa, Romero M.P.B., Albuquerque, Wendell Wagner Campos, Silva, Mariana C.C., Paula, Raiana Apolinário de, Melo, Mychely Sheila, Oliva, Maria L.V., Porto, Ana Lúcia Figueiredo
Format: Article
Language:English
Subjects:
Activity modulated
Amino Acid Sequence
Anticoagulants - chemistry
Anticoagulants - pharmacology
Binding site
Coagulation
Gamma radiation
Gamma Rays
Hemagglutination - drug effects
Humans
Loranthaceae - chemistry
Male
Plant Lectins - chemistry
Plant Lectins - pharmacology
Platelet aggregation
Platelet Aggregation - drug effects
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Summary:•Biological implications of proteins may be elucidated under radiation assays.•Lectin may be partial affected by low doses of gamma radiation.•Radiation modulates lectin anticoagulant activity.•A single binding site of PpyLL may be resistant to high radiation dose. Blood coagulation and platelet-dependent primary homeostasis are important defense mechanisms against bleeding and novel inhibitors have been researched to obtain pharmacological and clinical applications. In this work, the PpyLL, a lectin obtained from Phthirusa pyrifolia, was characterized in terms of its molecular structure and biological functions (anticoagulant, antiplatelet agreggation and hemagglutinating activities) in presence or absence of Gamma radiation exposure. Results revealed a lectin with secondary-structure content by approximately 49% of β-sheet, 20% of β-turn and 31% of disordered structure. Irradiation effect demonstrated possible different sites of function by lectin on anticoagulant and hemagglutinating activities, once a decrease about 80% was observed when compared the activities under 0.5kGy of exposition to gamma radiation. An emphatic discussion about the use of gamma radiation as a possible modulator of the lectin activity was made, and once the ionizing radiation affected differently the anticoagulation and hemagglutinating activities, we speculated that the results are determined by selective molecular damages in different binding sites. PpyLL biological activities and gamma radiation modulation could be considered for future researches in biomedical field aiming possible medical applications.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.06.007