Lysyl oxidase‐mediated VEGF‐induced differentiation and angiogenesis in human dental pulp cells

Aim To investigate the effects of recombinant human vascular endothelial growth factor (rhVEGF) on odontoblastic differentiation, in vitro angiogenesis, and expression and activity of lysyl oxidase (LOX) in human dental pulp cells (HDPCs), compared with rhFGF‐2. To identify the underlying molecular...

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Bibliographic Details
Published in:International endodontic journal 2018-03, Vol.51 (3), p.335-346
Main Authors: Bae, W‐J., Yi, J‐K., Park, J., Kang, S‐K., Jang, J‐H., Kim, E‐C.
Format: Article
Language:English
Subjects:
AKT protein
Alkaline phosphatase
Angiogenesis
Blotting, Western
Cell Differentiation - drug effects
Cell growth
Cell Line
Data processing
Dental pulp
Dental Pulp - cytology
Dental Pulp - drug effects
Dental Pulp - growth & development
Dentistry
differentiation
Endodontics
Gene silencing
human dental pulp cells
Humans
Immunocytochemistry
JNK protein
Lysyl oxidase
Mineralization
Molecular modelling
Neovascularization, Physiologic - drug effects
NF-κB protein
Nodules
Phosphorylation
Polymerase chain reaction
Protein-Lysine 6-Oxidase - antagonists & inhibitors
Protein-Lysine 6-Oxidase - metabolism
Recombinant Proteins
Reverse Transcriptase Polymerase Chain Reaction
Reverse transcription
RNA-mediated interference
Vascular endothelial growth factor
Vascular Endothelial Growth Factor A - pharmacology
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Summary:Aim To investigate the effects of recombinant human vascular endothelial growth factor (rhVEGF) on odontoblastic differentiation, in vitro angiogenesis, and expression and activity of lysyl oxidase (LOX) in human dental pulp cells (HDPCs), compared with rhFGF‐2. To identify the underlying molecular mechanisms, the study focused on whether LOX was responsible for the actions of rhVEGF. Methodology Recombinant human vascular endothelial growth factor (rhVEGF) was constructed using the pBAD‐HisA plasmid in Escherichia coli. HDPCs were treated with 1–50 μg mL−1 rhVEGF for 14 days. Alkaline phosphatase (ALP) activity was measured, and the formation of calcified nodules was assessed using alizarin red staining after the induction of odontogenic differentiation of HDPCs. The expression level of the odontogenic differentiation markers was detected by reverse transcription polymerase chain reaction. Signal pathways were assessed by Western blot and immunocytochemistry. The data were analysed by anova with Bonferroni's test (α = 0.05). Results Recombinant human vascular endothelial growth factor significantly increased cell growth (P 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.12796