Structure of a yeast spliceosome at 3.6-angstrom resolution

Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional struct...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2015-09, Vol.349 (6253), p.1182-1191
Main Authors: Yan, Chuangye, Hang, Jing, Wan, Ruixue, Huang, Min, Wong, Catherine C. L., Shi, Yigong
Format: Article
Language:English
Subjects:
Anchors
Catalysis
Catalysts
Catalytic Domain
Cryoelectron Microscopy
Mathematical models
Microbiology
Models, Molecular
Protein Structure, Secondary
Proteins
Repressor Proteins - chemistry
Ribonucleic acid
Ribonucleic acids
Ribonucleoprotein, U5 Small Nuclear - chemistry
RNA
RNA, Small Nuclear - chemistry
Saccharomyces cerevisiae
Schizosaccharomyces - ultrastructure
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - chemistry
Spliceosomes - chemistry
Spliceosomes - ultrastructure
Splicing
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.aac7629