L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells

In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quino...

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Bibliographic Details
Published in:Talanta (Oxford) 2017-08, Vol.171, p.16-24
Main Authors: Li, Zhenzhen, Zhang, Qiyi, Huang, Huaying, Ren, Changjing, Ouyang, Si, Zhao, Qiang
Format: Article
Language:English
Subjects:
Fluorescence quenching
Fluorescent Dyes - chemistry
Hep G2 Cells
Humans
Infrared Rays
NIR bioimaging
NIR probe
NIR QDs
Norepinephrine - chemistry
Optical Imaging - methods
Quantum Dots - chemistry
Urea - analysis
Urea - chemistry
Urea detection
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Summary:In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. [Display omitted] •The water-soluble CdSeTe QDs was functionalized with L-noradrenaline.•The functionalized probe have a fluorescence emission peak at 826nm.•The NIR probe was sensitive to pH value.•The NIR probe could determinate urea (0.057–13mmol/L) with urease.•The urea-bioimaging system could probe urea in the serum samples and HepG2 cells.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2017.04.003