High post-natal mortality associated with defects in lung maturation and reduced adiposity in mice with gestational exposure to high fat and N-acetylcysteine

Studies have demonstrated that maternal consumption of a high fat diet (HFD) increases offspring susceptibility to metabolic disease. This study was initiated to identify the mechanistic contribution of oxidative stress on this phenomenon. Two weeks prior to mating, dams were fed either HFD or Contr...

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Bibliographic Details
Published in:Research in veterinary science 2017-10, Vol.114, p.262-265
Main Authors: Williams, Lyda, Charron, Maureen J., Sellers, Rani S.
Format: Article
Language:English
Subjects:
Acetylcysteine
Acetylcysteine - adverse effects
Adipose tissue
Adipose tissue (brown)
Adiposity - drug effects
Animals
Breastfeeding & lactation
Carbohydrates
Diet
Diet, High-Fat - adverse effects
Dietary supplements
Drug dosages
Embryos
Female
Females
Gene expression
High fat diet
Insulin resistance
Lung
Lung - abnormalities
Lung - drug effects
Lung - growth & development
Lungs
Males
Mating
Maturation
Metabolism
Mice
Morphogenesis
Morphology
Mortality
Mouse
N-acetylcysteine
Neonates
Obesity
Offspring
Oxidative stress
Pregnancy
Prenatal Exposure Delayed Effects - chemically induced
Prenatal Exposure Delayed Effects - pathology
Prenatal Exposure Delayed Effects - physiopathology
Proteins
Rodents
Software
Studies
Veterinary medicine
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Summary:Studies have demonstrated that maternal consumption of a high fat diet (HFD) increases offspring susceptibility to metabolic disease. This study was initiated to identify the mechanistic contribution of oxidative stress on this phenomenon. Two weeks prior to mating, dams were fed either HFD or Control diet with or without supplementation with the anti-oxidant N-acetylcysteine (NAC). Pups born to HFD dams had reduced crown rump length (CRL) at birth and higher neonatal mortality compared to pups from Control dams. Supplementation with NAC normalized CRL in pups from HFD dams, but notably increased mortality. Histological examination of the lungs postnatally and prenatally, revealed normal branching morphogenesis but delayed alveolarization in pups from dams fed HFD+NAC. Discontinuation of NAC at ED17.5 with re-introduction at PD3 improved offspring survival and lung maturation. Additionally, interscapular brown adipose tissue (BAT) was reduced in ED18.5 embryos from HFD dams. These findings suggest that increased mortality in offspring from dams fed HFD+NAC during pregnancy may in part be the result of delayed pulmonary alveolarization and decreased BAT. •Maternal high fat diet (HFD) increases mortality in offspring.•Maternal HFD combined with N-acetylcysteine (NAC) markedly increases pup mortality.•Discontinuing NAC at ED17.5 and re-introducing at PD3 improved offspring survival.•Maternal HFD+NAC delayed pulmonary alveolarization but not branching morphogenesis.•Maternal HFD reduces offspring brown fat depots.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2017.05.020