Evaluation of the N-latex serum free light chain assay on the Siemens BNII analyzer and agreement with The Binding Site FreeLite assay on the SPAPlus

To evaluate the Siemens N-latex kappa free light chain (κFLC) and lambda FLC (λFLC) assays on the BNII nephelometer and assess agreement with The Binding Site Freelite FLC assays on the SPAPlus. Over 180 patient serum samples from routine analysis of κFLC and λFLC measured by the Freelite assay were...

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Bibliographic Details
Published in:Clinical biochemistry 2018-01, Vol.51, p.90-96
Main Authors: White-Al Habeeb, Nicole M.A., Earle, Tammy, Spencer, Megan, Blasutig, Ivan M.
Format: Article
Language:English
Subjects:
Freelite
Kappa FLC
Lambda FLC
N-latex
Serum free light chain
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Summary:To evaluate the Siemens N-latex kappa free light chain (κFLC) and lambda FLC (λFLC) assays on the BNII nephelometer and assess agreement with The Binding Site Freelite FLC assays on the SPAPlus. Over 180 patient serum samples from routine analysis of κFLC and λFLC measured by the Freelite assay were collected for the study and measured with the N-latex κFLC and λFLC assays to assess precision, linearity, method comparison and dilutional effects. Complex precision showed coefficients of variation of 4.8–7.2% for the κFLC assay and 3.6–6.0% for the λFLC assay. Linearity assessment showed both assays were linear (κFLC, y=1.00x−0.09 and λFLC, y=1.050x−1.252). Qualitative method comparison showed 87.9% (116/132) agreement and Cohen's kappa of 80.4% between the κFLC assays and 72.6% (98/135) agreement and Cohen's kappa of 55.4% for the λFLC assays. Quantitative method comparison for κFLC
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2017.05.009