Bioluminescent assay for toxicological assessment of nanomaterials

A new method for assessing biotoxicity of nanomaterials, based on the use of soluble bioluminescent coupled enzyme system NAD(P)⋅H:FMN oxidoreductase and luciferase, is proposed. The results of this study indicate a significant adverse biological effect exerted by nanoparticles at the molecular leve...

Full description

Saved in:
Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2017, Vol.472 (1), p.60-63
Main Authors: Esimbekova, E. N., Nemtseva, E. V., Kirillova, M. A., Asanova, A. A., Kratasyuk, V. A.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Bioluminescence
Biomedical and Life Sciences
Biophysics
Copper - chemistry
FMN Reductase - chemistry
Life Sciences
Luciferases - chemistry
Luminescent Measurements - methods
Metal Nanoparticles - chemistry
Metal Nanoparticles - toxicity
Molecular Biology
NADP - chemistry
Nanomaterials
Nanotubes, Carbon - chemistry
Nanotubes, Carbon - toxicity
Toxicity Tests - methods
Toxicology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A new method for assessing biotoxicity of nanomaterials, based on the use of soluble bioluminescent coupled enzyme system NAD(P)⋅H:FMN oxidoreductase and luciferase, is proposed. The results of this study indicate a significant adverse biological effect exerted by nanoparticles at the molecular level. It was found that the most toxic nanoparticles the nanoparticles are based on copper and copper oxide, as well as single-walled carbon nanotubes and multi-walled carbon nanofibers, which are referred to hazard class II.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672917010173