Effect of epigallocatechin-3-gallate on the increase in type II collagen accumulation in cartilage-like MSC sheets

With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) in...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2017-06, Vol.81 (6), p.1241-1245
Main Authors: Sato, Keigo, Mera, Hisashi, Wakitani, Shigeyuki, Takagi, Mutsumi
Format: Article
Language:English
Subjects:
Aged
Bone Marrow Cells - cytology
Bone Marrow Cells - drug effects
Bone Marrow Cells - metabolism
Cartilage - cytology
Cartilage - drug effects
Cartilage - metabolism
cartilage-like cell sheet
Catechin - analogs & derivatives
Catechin - pharmacology
Cell Differentiation - drug effects
Chondrocytes - cytology
Chondrocytes - drug effects
Chondrocytes - metabolism
Chondrogenesis - drug effects
Collagen Type II - agonists
Collagen Type II - genetics
Collagen Type II - metabolism
Culture Media - chemistry
Culture Media - pharmacology
epigallocatechin-3-gallate
Gene Expression Regulation
Humans
Male
Matrix Metalloproteinase 13 - genetics
Matrix Metalloproteinase 13 - metabolism
mesenchymal stem cell
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - metabolism
MMP-13
Primary Cell Culture
Proteolysis - drug effects
Reactive Oxygen Species - metabolism
SOX9 Transcription Factor - genetics
SOX9 Transcription Factor - metabolism
Tissue Engineering
type II collagen
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Summary:With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen. On the other hand, the addition of EGCG markedly decreased both the matrix metalloproteinase-13 concentration in the supernatant of cell sheet culture and the type II collagen degradation activity in that supernatant. Taken together, EGCG may enhance the accumulation of type II collagen by suppressing type II collagen degradation. Addition of EGCG to the sheet culture of MSC markedly increased not only wet weight (A) and sGAG amount (B) but also type II collagen amount (C) in the cartilage-like cell sheet.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2017.1282809