Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample

A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11 T , was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11 T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2017-08, Vol.110 (8), p.1027-1034
Main Authors: Zhu, Jie, Wang, Hui-Min, Zhang, Qi, Dong, Wei-Wei, Kong, De-Long, Wang, Yan-Wei, Song, Jin-Long, Jiang, Xu, Zhao, Shu-Miao, Zhang, Wei, Ruan, Zhi-Yong
Format: Article
Language:English
Subjects:
Alkanes
Bacteria
Bacteria - genetics
Bacteria - isolation & purification
Bacterial Typing Techniques
Base Composition
Biomedical and Life Sciences
China
Circularity
Deoxyribonucleic acid
DNA
DNA, Bacterial
Fatty Acids
Freezing
Frozen ground
Hybridization
Life Sciences
Lipids
Medical Microbiology
Microbiology
New species
Original Paper
Phosphatidylethanolamine
Phosphatidylglycerol
Phospholipids
Phylogeny
Plant Sciences
Quinones
RNA, Ribosomal, 16S
rRNA 16S
Samples
Sequence Analysis, DNA
Similarity
Sodium chloride
Soil
Soil Microbiology
Soil Science & Conservation
Soils
Ubiquinone
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Summary:A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11 T , was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11 T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11 T was found to be able to grow at 4–40 °C (optimum 15 °C), pH 7.5–2.0 (optimum 9.5) and 0–2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11 T is closely related to Arenimonas metalli CF5-1 T (98.0%), Arenimonas aquaticum NA-09 T (97.9%), Arenimonas donghaensis HO3-R19 T (95.6%) and Arenimonas aestuarii S2-21 T (95.3%). The DNA–DNA hybridization values between the isolate and A. metalli CGMCC 1.10787 T , A. aquaticum KACC 14663 T , A. donghaensis KACC 11381 T were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T m method. The major cellular fatty acids were identified as iso-C 15:0 and iso-C 16:0 . The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11 T is concluded to represent a novel species within the genus Arenimonas , for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11 T (ACCC 19750 T  = JCM 30464 T ).
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-017-0876-9