Reference intervals for serum 24,25-dihydroxyvitamin D and the ratio with 25-hydroxyvitamin D established using a newly developed LC–MS/MS method

24,25(OH)2D is the product of 25(OH)D catabolism by CYP24A1. The measurement of serum 24,25(OH)2D concentration may serve as an indicator of vitamin D catabolic status and the relative ratio with 25(OH)D can be used to identify patients with inactivating mutations in CYP24A1. We describe a LC–MS/MS...

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Bibliographic Details
Published in:The Journal of nutritional biochemistry 2017-08, Vol.46, p.21-29
Main Authors: Tang, Jonathan C.Y., Nicholls, Holly, Piec, Isabelle, Washbourne, Christopher J., Dutton, John J., Jackson, Sarah, Greeves, Julie, Fraser, William D.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Calcium - blood
Child
Child, Preschool
Chromatography, Liquid - methods
Female
Humans
Hypercalcemia - blood
Hypercalcemia - genetics
Male
Middle Aged
Reference Values
Reproducibility of Results
Tandem Mass Spectrometry - methods
Vitamin D - analogs & derivatives
Vitamin D - blood
Vitamin D Deficiency - blood
Vitamin D3 24-Hydroxylase - genetics
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Summary:24,25(OH)2D is the product of 25(OH)D catabolism by CYP24A1. The measurement of serum 24,25(OH)2D concentration may serve as an indicator of vitamin D catabolic status and the relative ratio with 25(OH)D can be used to identify patients with inactivating mutations in CYP24A1. We describe a LC–MS/MS method to determine: (1) the relationships between serum 24,25(OH)2D and 25(OH)D; (2) serum reference intervals in healthy individuals; (3) the diagnostic accuracy of 24,25(OH)2D measurement as an indicator for vitamin D status; 4) 24,25(OH)2D cut-off value for clinically significant change between inadequate and sufficient 25(OH)D status. Serum samples of healthy participants (n=1996) from Army recruits and patients (n=294) were analysed. The LC–MS/MS assay satisfied industry standards for method validation. We found a positive, concentration-dependent relationship between serum 24,25(OH)2D and 25(OH)2D concentrations. The 25(OH)D:24,25(OH)2D ratio was significantly higher (P
ISSN:0955-2863
1873-4847
DOI:10.1016/j.jnutbio.2017.04.005