Genome sequence of bubaline alphaherpesvirus 1 (BuHV1) isolated in Australia in 1972

Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae , subfamily Alphaherpesvirinae , genus Varicellovirus . To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), i...

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Bibliographic Details
Published in:Archives of virology 2017-05, Vol.162 (5), p.1169-1176
Main Authors: Scheffer, Camila Mengue, Varela, Ana Paula Muterle, Cibulski, Samuel Paulo, Schmidt, Candice, Campos, Fabrício Souza, Paim, Willian Pinto, dos Santos, Raíssa Nunes, Teixeira, Thais Fumaco, Loiko, Márcia Regina, Tochetto, Caroline, dos Santos, Helton Fernandes, de Lima, Diane Alves, Cerva, Cristine, Mayer, Fabiana Quoos, Petzhold, Sylio Alfredo, Franco, Ana Cláudia, George, Toby St, Spilki, Fernando Rosado, Roehe, Paulo Michel
Format: Article
Language:English
Subjects:
Alphaherpesvirinae
Animals
Australia
Base Sequence
Biomedical and Life Sciences
Biomedicine
Bubalus bubalis
Buffalo
Buffaloes - virology
Cattle
Cell Line
DNA, Viral - genetics
Dogs
Genome, Viral - genetics
Genomes
Health sciences
Herpes viruses
Herpesviridae
High-Throughput Nucleotide Sequencing
Infectious Diseases
Madin Darby Canine Kidney Cells
Medical Microbiology
Original Article
Sequence Analysis, DNA
Steroids
Varicellovirus
Varicellovirus - classification
Varicellovirus - genetics
Varicellovirus - isolation & purification
Virology
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Summary:Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae , subfamily Alphaherpesvirinae , genus Varicellovirus . To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water buffalo ( Bubalus bubalis ) in 1972 in Australia. The virus was multiplied in MDBK cells, and the DNA was extracted and subjected to high-throughput sequencing. The reads were aligned and combined into a single genome sequence, with bovine alphaherpesvirus 5 (BoHV5) strain SV507/99 (accession number NC005261) as a reference. The BuHV1-b6 genome is a linear double-stranded DNA molecule, 137,452 bp long, with a GC content of 76.8%. The genome consists of two unique sequences: a long, or UL, sequence (103,818 bp) and a short, or US, sequence (9,586 bp), with the latter being flanked by inverted IR and TR elements of 12,024 bp each. The arrangement is typical of herpesvirus genomes of the D-type. The overall sequence has a 92.2% similarity at the nucleotide level to the reference BoHV5 strain. Our report provides a significant landmark in the history of herpesviruses, represented by the genome sequence of this 44-year-old virus isolate.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-3218-8