Structure and gene cluster of the K93 capsular polysaccharide of Acinetobacter baumannii B11911 containing 5-N-Acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid

Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1 H and 13 C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2017-04, Vol.82 (4), p.483-489
Main Authors: Kasimova, A. A., Shneider, M. M., Arbatsky, N. P., Popova, A. V., Shashkov, A. S., Miroshnikov, K. A., Balaji, Veeraraghavan, Biswas, Indranil, Knirel, Yu. A.
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter baumannii
Acinetobacter baumannii - genetics
Acinetobacter baumannii - metabolism
Bacterial Capsules - metabolism
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Biosynthesis
Carbohydrate Conformation
Carbon-13 Magnetic Resonance Spectroscopy
Gene expression
Genes, Bacterial
Genetic aspects
Life Sciences
Microbiology
Multigene Family
NMR
Nuclear magnetic resonance
Observations
Physiological aspects
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - genetics
Proton Magnetic Resonance Spectroscopy
Sugar Acids - analysis
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Summary:Capsular polysaccharide (CPS) assigned to the K93 type was isolated from the bacterium Acinetobacter baumannii B11911 and studied by sugar analysis along with oneand two-dimensional 1 H and 13 C NMR spectroscopy. The CPS was found to contain a derivative of pseudaminic acid, and the structure of the branched tetrasaccharide repeating unit was established. Genes in the KL93 capsule biosynthesis locus were annotated and found to be consistent with the CPS structure established. The K93 CPS has the α-D-Gal p -(1→6)-β-D-Gal p -(1→3)-D-Gal p NAc trisaccharide fragment in common with the K14 CPS of Acinetobacter nosocomialis LUH 5541 and A. baumannii D46. It also shares the β-D-Gal p -(1→3)-DGal p NAc disaccharide fragment and the corresponding predicted Gal transferase Gtr5, as well as the initiating GalNAc-1P transferase ItrA2, with a number of A. baumannii strains.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297917040101