Effects of trimethylsilane plasma coating on the hydrophobicity of denture base resin and adhesion of Candida albicans on resin surfaces

Abstract Statement of problem Candida -associated denture stomatitis is the most common oral mucosal lesion among denture wearers. Trimethylsilane (TMS) plasma coating may inhibit the growth of Candida albicans on denture surfaces. Purpose The purpose of this in vitro study was to investigate whethe...

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Bibliographic Details
Published in:The Journal of prosthetic dentistry 2017-12, Vol.118 (6), p.765-770
Main Authors: Liu, Tianshuang, DDS, Xu, Changqi, PhD, Hong, Liang, DDS, PhD, Garcia-Godoy, Franklin, DDS, PhD, Hottel, Timothy, DDS, MS, MBA, Babu, Jegdish, PhD, Yu, Qingsong, PhD
Format: Article
Language:English
Subjects:
Dentistry
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Summary:Abstract Statement of problem Candida -associated denture stomatitis is the most common oral mucosal lesion among denture wearers. Trimethylsilane (TMS) plasma coating may inhibit the growth of Candida albicans on denture surfaces. Purpose The purpose of this in vitro study was to investigate whether TMS plasma coatings can effectively reduce C albicans adhesion on denture base acrylic resin surfaces. Material and methods Sixty denture base acrylic resin disks with smooth and rough surfaces were prepared and were either left untreated (control group) or coated with TMS monomer (experimental group) by using plasma. Contact angles were measured immediately after TMS plasma coating. The morphology of C albicans adhesion was observed with scanning electron microscopy (SEM). Energy-dispersive spectroscopy (EDS) was used to characterize the elemental composition of the specimen surface. An adhesion test was performed by incubating the resin disk specimens in C albicans suspensions (1×107 cells/mL) at 37°C for 24 hours and further measuring the optical density of the C albicans by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay test. One-way ANOVA and 2-way ANOVA were followed by a post hoc test analysis (α=.05). Results The group with TMS coating exhibited a more hydrophobic surface than the control group. EDS analysis revealed successful TMS plasma coating. The difference in the mean contact angles between the uncoated group and the TMS-coated group was statistically significant ( P
ISSN:0022-3913
1097-6841
DOI:10.1016/j.prosdent.2017.01.003