Establishing alighting preferences and species transmission differences for Pea seed-borne mosaic virus aphid vectors

•This study provides important new information which enhances understanding of virus-vector relationships in the PSbMV-pea pathosystem.•It found that A. craccivora and M. persicae were efficient vectors, but A. kondoi and R. padi were inefficient. L. erysimi did not transmit.•R. padi alatae preferen...

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Bibliographic Details
Published in:Virus research 2017-09, Vol.241, p.145-155
Main Authors: Congdon, B.S., Coutts, B.A., Renton, M., Flematti, G.R., Jones, R.A.C.
Format: Article
Language:English
Subjects:
Alighting preference
Animals
Aphid
Aphids - virology
Australia
Insect Vectors - virology
Manipulation
Non-persistent
Pisum sativum - virology
Plant Diseases - virology
Potyvirus - physiology
PSbMV
Transmission efficiency
Volatile Organic Compounds - metabolism
Volatiles
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Summary:•This study provides important new information which enhances understanding of virus-vector relationships in the PSbMV-pea pathosystem.•It found that A. craccivora and M. persicae were efficient vectors, but A. kondoi and R. padi were inefficient. L. erysimi did not transmit.•R. padi alatae preferentially alighted on infected plants early on, followed by a shift to mock-inoculated plants after 24h.•In contrast, M. persicae alatae exhibited a preference to alight on mock-inoculated at all times tested.•PSbMV-induced alterations to plant VOC profiles could be responsible for aphid attraction or repulsion to infected plants. Pea seed-borne mosaic virus (PSbMV) infection causes a serious disease of field pea (Pisum sativum) crops worldwide. The PSbMV transmission efficiencies of five aphid species previously found landing in south-west Australian pea crops in which PSbMV was spreading were studied. With plants of susceptible pea cv. Kaspa, the transmission efficiencies of Aphis craccivora, Myzus persicae, Acyrthosiphon kondoi and Rhopalosiphum padi were 27%, 26%, 6% and 3%, respectively. Lipaphis erysimi did not transmit PSbMV in these experiments. The transmission efficiencies found for M. persicae and A. craccivora resembled earlier findings, but PSbMV vector transmission efficiency data were unavailable for A. kondoi, R. padi and L. erysimi. With plants of partially PSbMV resistant pea cv. PBA Twilight, transmission efficiencies of M. persicae, A. craccivora and R. padi were 16%, 12% and 1%, respectively, reflecting putative partial resistance to aphid inoculation. To examine aphid alighting preferences over time, free-choice assays were conducted with two aphid species representing efficient (M. persicae) and inefficient (R. padi) vector species. For this, alatae were set free on multiple occasions (10–15 repetitions each) amongst PSbMV-infected and mock-inoculated pea or faba bean (Vicia faba) plants. Following release, non-viruliferous R. padi alatae exhibited a general preference for PSbMV-infected pea and faba bean plants after 30min–4h, but preferred mock-inoculated plants after 24h. In contrast, non-viruliferous M. persicae alatae alighted on mock-inoculated pea plants preferentially for up to 48h following their release. With faba bean, M. persicae preferred infected plants at the front of assay cages, but mock-inoculated ones their backs, apparently due to increased levels of natural light there. When preliminary analyses were performed to detect P
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2017.04.005