Inhibitory effect of Thymus vulgaris and Origanum vulgare essential oils on virulence factors of phytopathogenic Pseudomonas syringae strains

Pseudomonas syringae is a phytopathogenic bacterium that causes lesions in leaves during the colonisation process. The damage is associated with production of many virulence factors, such as biofilm and phytotoxins. The essential oils of Thymus vulgaris (thyme) and Origanum vulgare (oregano) have be...

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Bibliographic Details
Published in:Plant biology (Stuttgart, Germany) Germany), 2017-07, Vol.19 (4), p.599-607
Main Authors: Carezzano, M E, Sotelo, J P, Primo, E, Reinoso, E B, Paletti Rovey, M F, Demo, M S, Giordano, W F, Oliva, M de Las M
Format: Article
Language:English
Subjects:
Amino Acids - metabolism
Antibacterial activity
Bacterial Proteins - metabolism
Bactericidal activity
Biofilms
Colonization
Coronatine
Dipeptides - metabolism
Essential oils
Indenes - metabolism
Lesions
Microbial Sensitivity Tests
Minimum inhibitory concentration
Natural products
Oils & fats
Oils, Volatile - chemistry
Oils, Volatile - pharmacology
Oregano
Origanum - chemistry
Origanum vulgare
Pathogens
Phytotoxins
Plant Extracts - pharmacology
Pseudomonas
Pseudomonas syringae
Pseudomonas syringae - drug effects
Pseudomonas syringae - metabolism
Spices
Strains (organisms)
Thyme
Thymus Plant - chemistry
Thymus vulgaris
Toxins
Virulence
Virulence factors
Virulence Factors - metabolism
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Summary:Pseudomonas syringae is a phytopathogenic bacterium that causes lesions in leaves during the colonisation process. The damage is associated with production of many virulence factors, such as biofilm and phytotoxins. The essential oils of Thymus vulgaris (thyme) and Origanum vulgare (oregano) have been demonstrated to inhibit P. syringae. The aim of this study was to investigate the effects of T. vulgaris and O. vulgare essential oils on production of virulence factors of phytopathogenic P. syringae strains, including anti-biofilm and anti-toxins activities. The broth microdilution method was used for determination of MIC and biofilm inhibition assays. Coronatine, syringomycin and tabtoxin were pheno- and genotypically evaluated. Both oils showed good inhibitory activity against P. syringae, with MIC values from 1.43 to 11.5 mg·ml for thyme and 5.8 to 11.6 mg·ml for oregano. Biofilm formation, production of coronatine, syringomycin and tabtoxin were inhibited by thyme and oregano essential oil in most strains. The results presented here are promising, demonstrating the bactericidal activity and reduction of virulence factor production after treatment with thyme and oregano oil, providing insight into how they exert their antibacterial activity. These natural products could be considered in the future for the control of diseases caused by P. syringae.
ISSN:1435-8603
1438-8677
DOI:10.1111/plb.12572