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Exfoliated Human Olfactory Neuroepithelium: A Source of Neural Progenitor Cells
Neural progenitor cells (NPC) contained in the human adult olfactory neuroepithelium (ONE) possess an undifferentiated state, the capability of self-renewal, the ability to generate neural and glial cells as well as being kept as neurospheres in cell culture conditions. Recently, NPC have been isola...
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| Published in: | Molecular neurobiology 2018-03, Vol.55 (3), p.2516-2523 |
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| creator | Jiménez-Vaca, Ana L. Benitez-King, Gloria Ruiz, Víctor Ramírez-Rodríguez, Gerardo B. Hernández-de la Cruz, Beatriz Salamanca-Gómez, Fabio A. González-Márquez, Humberto Ramírez-Sánchez, Israel Ortíz-López, Leonardo Vélez-del Valle, Cristina Ordoñez-Razo, Rosa Ma |
| description | Neural progenitor cells (NPC) contained in the human adult olfactory neuroepithelium (ONE) possess an undifferentiated state, the capability of self-renewal, the ability to generate neural and glial cells as well as being kept as neurospheres in cell culture conditions. Recently, NPC have been isolated from human or animal models using high-risk surgical methods. Therefore, it was necessary to improve methodologies to obtain and maintain human NPC as well as to achieve better knowledge of brain disorders. In this study, we propose the establishment and characterization of NPC cultures derived from the human olfactory neuroepithelium, using non-invasive procedures. Twenty-two healthy individuals (29.7 ± 4.5 years of age) were subjected to nasal exfoliation. Cells were recovered and kept as neurospheres under serum-free conditions. The neural progenitor origin of these neurospheres was determined by immunocytochemistry and qPCR. Their ability for self-renewal and multipotency was analyzed by clonogenic and differentiation assays, respectively. In the cultures, the ONE cells preserved the phenotype of the neurospheres. The expression levels of Nestin, Musashi, Sox2, and βIII-tubulin demonstrated the neural origin of the neurospheres; 48% of the cells separated could generate neurospheres, determining that they retained their self-renewal capacity. Neurospheres were differentiated in the absence of growth factors (EGF and FGF), and their multipotency ability was maintained as well. We were also able to isolate and grow human neural progenitor cells (neurospheres) through nasal exfoliates (non-invasive method) of the ONE from healthy adults, which is an extremely important contribution for the study of brain disorders and for the development of new therapies. |
| doi_str_mv | 10.1007/s12035-017-0500-z |
| format | article |
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Recently, NPC have been isolated from human or animal models using high-risk surgical methods. Therefore, it was necessary to improve methodologies to obtain and maintain human NPC as well as to achieve better knowledge of brain disorders. In this study, we propose the establishment and characterization of NPC cultures derived from the human olfactory neuroepithelium, using non-invasive procedures. Twenty-two healthy individuals (29.7 ± 4.5 years of age) were subjected to nasal exfoliation. Cells were recovered and kept as neurospheres under serum-free conditions. The neural progenitor origin of these neurospheres was determined by immunocytochemistry and qPCR. Their ability for self-renewal and multipotency was analyzed by clonogenic and differentiation assays, respectively. In the cultures, the ONE cells preserved the phenotype of the neurospheres. The expression levels of Nestin, Musashi, Sox2, and βIII-tubulin demonstrated the neural origin of the neurospheres; 48% of the cells separated could generate neurospheres, determining that they retained their self-renewal capacity. Neurospheres were differentiated in the absence of growth factors (EGF and FGF), and their multipotency ability was maintained as well. We were also able to isolate and grow human neural progenitor cells (neurospheres) through nasal exfoliates (non-invasive method) of the ONE from healthy adults, which is an extremely important contribution for the study of brain disorders and for the development of new therapies.</description><identifier>ISSN: 0893-7648</identifier><identifier>EISSN: 1559-1182</identifier><identifier>DOI: 10.1007/s12035-017-0500-z</identifier><identifier>PMID: 28391555</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adult ; Animal models ; Biomedical and Life Sciences ; Biomedicine ; Brain ; Cell Biology ; Cell culture ; Cell self-renewal ; Cells, Cultured ; Female ; Fibroblast growth factors ; Glial cells ; Growth factors ; Humans ; Immunocytochemistry ; Male ; Nestin ; Neural stem cells ; Neural Stem Cells - physiology ; Neurobiology ; Neuroepithelial Cells - physiology ; Neurology ; Neuronal-glial interactions ; Neurosciences ; Neurospheres ; Olfactory epithelium ; Olfactory Mucosa - cytology ; Olfactory Mucosa - physiology ; Phenotypes ; Progenitor cells ; Tubulin</subject><ispartof>Molecular neurobiology, 2018-03, Vol.55 (3), p.2516-2523</ispartof><rights>Springer Science+Business Media New York 2017</rights><rights>Molecular Neurobiology is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-5527fd5aee15ebf2cdbecfd2289117a760fd0519750c0d80f7a96002a71676323</citedby><cites>FETCH-LOGICAL-c372t-5527fd5aee15ebf2cdbecfd2289117a760fd0519750c0d80f7a96002a71676323</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28391555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiménez-Vaca, Ana L.</creatorcontrib><creatorcontrib>Benitez-King, Gloria</creatorcontrib><creatorcontrib>Ruiz, Víctor</creatorcontrib><creatorcontrib>Ramírez-Rodríguez, Gerardo B.</creatorcontrib><creatorcontrib>Hernández-de la Cruz, Beatriz</creatorcontrib><creatorcontrib>Salamanca-Gómez, Fabio A.</creatorcontrib><creatorcontrib>González-Márquez, Humberto</creatorcontrib><creatorcontrib>Ramírez-Sánchez, Israel</creatorcontrib><creatorcontrib>Ortíz-López, Leonardo</creatorcontrib><creatorcontrib>Vélez-del Valle, Cristina</creatorcontrib><creatorcontrib>Ordoñez-Razo, Rosa Ma</creatorcontrib><title>Exfoliated Human Olfactory Neuroepithelium: A Source of Neural Progenitor Cells</title><title>Molecular neurobiology</title><addtitle>Mol Neurobiol</addtitle><addtitle>Mol Neurobiol</addtitle><description>Neural progenitor cells (NPC) contained in the human adult olfactory neuroepithelium (ONE) possess an undifferentiated state, the capability of self-renewal, the ability to generate neural and glial cells as well as being kept as neurospheres in cell culture conditions. Recently, NPC have been isolated from human or animal models using high-risk surgical methods. Therefore, it was necessary to improve methodologies to obtain and maintain human NPC as well as to achieve better knowledge of brain disorders. In this study, we propose the establishment and characterization of NPC cultures derived from the human olfactory neuroepithelium, using non-invasive procedures. Twenty-two healthy individuals (29.7 ± 4.5 years of age) were subjected to nasal exfoliation. Cells were recovered and kept as neurospheres under serum-free conditions. The neural progenitor origin of these neurospheres was determined by immunocytochemistry and qPCR. Their ability for self-renewal and multipotency was analyzed by clonogenic and differentiation assays, respectively. In the cultures, the ONE cells preserved the phenotype of the neurospheres. The expression levels of Nestin, Musashi, Sox2, and βIII-tubulin demonstrated the neural origin of the neurospheres; 48% of the cells separated could generate neurospheres, determining that they retained their self-renewal capacity. Neurospheres were differentiated in the absence of growth factors (EGF and FGF), and their multipotency ability was maintained as well. We were also able to isolate and grow human neural progenitor cells (neurospheres) through nasal exfoliates (non-invasive method) of the ONE from healthy adults, which is an extremely important contribution for the study of brain disorders and for the development of new therapies.</description><subject>Adult</subject><subject>Animal models</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell self-renewal</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fibroblast growth factors</subject><subject>Glial cells</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Immunocytochemistry</subject><subject>Male</subject><subject>Nestin</subject><subject>Neural stem cells</subject><subject>Neural Stem Cells - physiology</subject><subject>Neurobiology</subject><subject>Neuroepithelial Cells - physiology</subject><subject>Neurology</subject><subject>Neuronal-glial interactions</subject><subject>Neurosciences</subject><subject>Neurospheres</subject><subject>Olfactory epithelium</subject><subject>Olfactory Mucosa - 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Academic</collection><jtitle>Molecular neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiménez-Vaca, Ana L.</au><au>Benitez-King, Gloria</au><au>Ruiz, Víctor</au><au>Ramírez-Rodríguez, Gerardo B.</au><au>Hernández-de la Cruz, Beatriz</au><au>Salamanca-Gómez, Fabio A.</au><au>González-Márquez, Humberto</au><au>Ramírez-Sánchez, Israel</au><au>Ortíz-López, Leonardo</au><au>Vélez-del Valle, Cristina</au><au>Ordoñez-Razo, Rosa Ma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exfoliated Human Olfactory Neuroepithelium: A Source of Neural Progenitor Cells</atitle><jtitle>Molecular neurobiology</jtitle><stitle>Mol Neurobiol</stitle><addtitle>Mol Neurobiol</addtitle><date>2018-03-01</date><risdate>2018</risdate><volume>55</volume><issue>3</issue><spage>2516</spage><epage>2523</epage><pages>2516-2523</pages><issn>0893-7648</issn><eissn>1559-1182</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Neural progenitor cells (NPC) contained in the human adult olfactory neuroepithelium (ONE) possess an undifferentiated state, the capability of self-renewal, the ability to generate neural and glial cells as well as being kept as neurospheres in cell culture conditions. Recently, NPC have been isolated from human or animal models using high-risk surgical methods. Therefore, it was necessary to improve methodologies to obtain and maintain human NPC as well as to achieve better knowledge of brain disorders. In this study, we propose the establishment and characterization of NPC cultures derived from the human olfactory neuroepithelium, using non-invasive procedures. Twenty-two healthy individuals (29.7 ± 4.5 years of age) were subjected to nasal exfoliation. Cells were recovered and kept as neurospheres under serum-free conditions. The neural progenitor origin of these neurospheres was determined by immunocytochemistry and qPCR. Their ability for self-renewal and multipotency was analyzed by clonogenic and differentiation assays, respectively. In the cultures, the ONE cells preserved the phenotype of the neurospheres. The expression levels of Nestin, Musashi, Sox2, and βIII-tubulin demonstrated the neural origin of the neurospheres; 48% of the cells separated could generate neurospheres, determining that they retained their self-renewal capacity. Neurospheres were differentiated in the absence of growth factors (EGF and FGF), and their multipotency ability was maintained as well. We were also able to isolate and grow human neural progenitor cells (neurospheres) through nasal exfoliates (non-invasive method) of the ONE from healthy adults, which is an extremely important contribution for the study of brain disorders and for the development of new therapies.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>28391555</pmid><doi>10.1007/s12035-017-0500-z</doi><tpages>8</tpages></addata></record> |
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| subjects | Adult Animal models Biomedical and Life Sciences Biomedicine Brain Cell Biology Cell culture Cell self-renewal Cells, Cultured Female Fibroblast growth factors Glial cells Growth factors Humans Immunocytochemistry Male Nestin Neural stem cells Neural Stem Cells - physiology Neurobiology Neuroepithelial Cells - physiology Neurology Neuronal-glial interactions Neurosciences Neurospheres Olfactory epithelium Olfactory Mucosa - cytology Olfactory Mucosa - physiology Phenotypes Progenitor cells Tubulin |
| title | Exfoliated Human Olfactory Neuroepithelium: A Source of Neural Progenitor Cells |
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