Exfoliated Human Olfactory Neuroepithelium: A Source of Neural Progenitor Cells

Neural progenitor cells (NPC) contained in the human adult olfactory neuroepithelium (ONE) possess an undifferentiated state, the capability of self-renewal, the ability to generate neural and glial cells as well as being kept as neurospheres in cell culture conditions. Recently, NPC have been isola...

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Published in:Molecular neurobiology 2018-03, Vol.55 (3), p.2516-2523
Main Authors: Jiménez-Vaca, Ana L., Benitez-King, Gloria, Ruiz, Víctor, Ramírez-Rodríguez, Gerardo B., Hernández-de la Cruz, Beatriz, Salamanca-Gómez, Fabio A., González-Márquez, Humberto, Ramírez-Sánchez, Israel, Ortíz-López, Leonardo, Vélez-del Valle, Cristina, Ordoñez-Razo, Rosa Ma
Format: Article
Language:English
Subjects:
Adult
Animal models
Biomedical and Life Sciences
Biomedicine
Brain
Cell Biology
Cell culture
Cell self-renewal
Cells, Cultured
Female
Fibroblast growth factors
Glial cells
Growth factors
Humans
Immunocytochemistry
Male
Nestin
Neural stem cells
Neural Stem Cells - physiology
Neurobiology
Neuroepithelial Cells - physiology
Neurology
Neuronal-glial interactions
Neurosciences
Neurospheres
Olfactory epithelium
Olfactory Mucosa - cytology
Olfactory Mucosa - physiology
Phenotypes
Progenitor cells
Tubulin
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Summary:Neural progenitor cells (NPC) contained in the human adult olfactory neuroepithelium (ONE) possess an undifferentiated state, the capability of self-renewal, the ability to generate neural and glial cells as well as being kept as neurospheres in cell culture conditions. Recently, NPC have been isolated from human or animal models using high-risk surgical methods. Therefore, it was necessary to improve methodologies to obtain and maintain human NPC as well as to achieve better knowledge of brain disorders. In this study, we propose the establishment and characterization of NPC cultures derived from the human olfactory neuroepithelium, using non-invasive procedures. Twenty-two healthy individuals (29.7 ± 4.5 years of age) were subjected to nasal exfoliation. Cells were recovered and kept as neurospheres under serum-free conditions. The neural progenitor origin of these neurospheres was determined by immunocytochemistry and qPCR. Their ability for self-renewal and multipotency was analyzed by clonogenic and differentiation assays, respectively. In the cultures, the ONE cells preserved the phenotype of the neurospheres. The expression levels of Nestin, Musashi, Sox2, and βIII-tubulin demonstrated the neural origin of the neurospheres; 48% of the cells separated could generate neurospheres, determining that they retained their self-renewal capacity. Neurospheres were differentiated in the absence of growth factors (EGF and FGF), and their multipotency ability was maintained as well. We were also able to isolate and grow human neural progenitor cells (neurospheres) through nasal exfoliates (non-invasive method) of the ONE from healthy adults, which is an extremely important contribution for the study of brain disorders and for the development of new therapies.
ISSN:0893-7648
1559-1182
DOI:10.1007/s12035-017-0500-z