Idiopathic atrophoderma of Pasini and Pierini: A case study of collagen and elastin texture by multiphoton microscopy

Background The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton i...

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Bibliographic Details
Published in:Journal of the American Academy of Dermatology 2017-11, Vol.77 (5), p.930-937
Main Authors: Vieira-Damiani, Gislaine, PhD, Lage, Denise, PhD, Christofoletti Daldon, Patrícia Érica, PhD, Tibúrcio Alves, Caroline Romanelli, MD, Cintra, Maria Letícia, PhD, Metze, Konradin, PhD, Adur, Javier, PhD, Pelegati, Vitor B., PhD, Carvalho, Hernandes F., PhD, Cesar, Carlos Lenz, PhD
Format: Article
Language:English
Subjects:
2-photon excited fluorescence
Adolescent
Adult
atrophoderma
atrophy
Atrophy - pathology
Biopsy, Needle
Collagen - ultrastructure
collagen fibers
Dermatology
elastic fibers
Elastic Tissue - pathology
Elastic Tissue - ultrastructure
Female
Humans
idiopathic atrophoderma of Pasini and Pierini
Immunohistochemistry
Male
Microscopy, Fluorescence, Multiphoton - methods
Middle Aged
morphometry
pathology
Reference Values
Sampling Studies
scleroderma
Scleroderma, Localized - diagnostic imaging
Scleroderma, Localized - pathology
second harmonic generation
SHG
Statistics, Nonparametric
TPEF
Young Adult
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Summary:Background The diagnosis of idiopathic atrophoderma of Pasini and Pierini (IAPP) relies on typical clinical features, particularly distinctive pigmented ovular/round depressed plaques. Histologic examination often reveals no obvious changes, but patterns of collagen distribution, using multiphoton imaging and second harmonic generation can help track hidden details of tissue organization contributing to atrophy. Objective To identify histologic features that distinguish IAPP from unaffected skin. Methods Eleven patients were included for conventional analyses. Masson trichrome– and Unna-Tanzer orcein–stained sections were evaluated using automated morphometry. Hematoxylin-eosin–stained sections were analyzed by multiphoton imaging using 2-photon excited fluorescence and second harmonic generation. Results No abnormalities were found under light microscopy or by automated quantification. Multiphoton imaging revealed no difference in optical density of either collagen or elastic fibers in lesioned and unaffected skin; however, horizontal collagen fiber organization in lesion specimens increased toward the lower dermis, whereas elastic fibers featured greater disorganization within the upper dermis. Limitations The low number of patients evaluated. Conclusion The atrophic appearance of IAPP lesions reflects changes in organization, but not in collagen and elastic tissue content. Minute organizational differences that are imperceptible to the experienced pathologist and undetectable by automated analyses were revealed by multiphoton analyses, particularly second harmonic generation, in association with texture analyses.
ISSN:0190-9622
1097-6787
DOI:10.1016/j.jaad.2017.02.044