Endurance training affects myosin heavy chain phenotype in regenerating fast-twitch muscle

Xavier A. Bigard, Chantal Janmot, Danièle Merino, Françoise Lienhard, Yannick C. Guezennec, and Anne D'Albis Département de Physiologie Systémique, Institut de Médecine Aésopatiale du Service de Santé des Acmées, 91223 Brétigny sur Orge cedex; and Laboratoire de Biologie Physicochimique, Unité...

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Published in:Journal of applied physiology (1985) 1996-12, Vol.81 (6), p.2658-2665
Main Authors: Bigard, Xavier A, Janmot, Chantal, Merino, Daniele, Lienhard, Francoise, Guezennec, Yannick C, D'Albis, Anne
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Male
Muscle Fibers, Fast-Twitch - metabolism
Myosin Heavy Chains - metabolism
Physical Conditioning, Animal - physiology
Rats
Rats, Wistar
Regeneration - physiology
Space life sciences
Striated muscle. Tendons
Vertebrates: osteoarticular system, musculoskeletal system
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Summary:Xavier A. Bigard, Chantal Janmot, Danièle Merino, Françoise Lienhard, Yannick C. Guezennec, and Anne D'Albis Département de Physiologie Systémique, Institut de Médecine Aésopatiale du Service de Santé des Acmées, 91223 Brétigny sur Orge cedex; and Laboratoire de Biologie Physicochimique, Unité de Recherche Associée, Centre National de la Recherche Scientifique 1131, Université Paris Sud, 91405 Orsay, France Received 16 April 1996; accepted in final form 13 August 1996. Bigard, Xavier A., Chantal Janmot, Danièle Merino, Françoise Lienhard, Yannick C. Guezennec, and Anne D'Albis. Endurance training affects myosin heavy chain phenotype in regenerating fast-twitch muscle. J. Appl. Physiol. 81(6): 2658-2665, 1996. The aim of this study was to analyze the effects of treadmill training (2 h/day, 5 days/wk, 30 m/min, 7% grade for 5 wk) on the expression of myosin heavy chain (MHC) isoforms during and after regeneration of a fast-twitch white muscle [extensor digitorum longus (EDL)]. Male Wistar rats were randomly assigned to a sedentary ( n  = 10) or an endurance-trained (ET; n  = 10) group. EDL muscle degeneration and regeneration were induced by two subcutaneous injections of a snake toxin. Five days after induction of muscle injury, animals were trained over a 5-wk period. It was verified that ~40 days after venom treatment, central nuclei were present in the treated EDL muscles from sedentary and ET rats. The changes in the expression of MHCs in EDL muscles were detected by using a combination of biochemical and immunocytochemical approaches. Compared with contralateral nondegenerated muscles, relative concentrations of types I, IIa, and IIx MHC isoforms in ET rats were greater in regenerated EDL muscles (146%, P  
ISSN:8750-7587
1522-1601
DOI:10.1152/jappl.1996.81.6.2658