Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia

ABSTRACT H5 highly pathogenic avian influenza viruses (HPAIV) have spread in both poultry and wild birds since late 2003. Continued circulation of HPAIV in poultry in several regions of the world has led to antigenic drift. In the present study, we analyzed the antigenic properties of H5 HPAIV isola...

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Bibliographic Details
Published in:Microbiology and immunology 2017-05, Vol.61 (5), p.149-158
Main Authors: Ohkawara, Ayako, Okamatsu, Masatoshi, Ozawa, Makoto, Chu, Duc‐Huy, Nguyen, Lam Thanh, Hiono, Takahiro, Matsuno, Keita, Kida, Hiroshi, Sakoda, Yoshihiro
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antigenic drift
Antigenic Variation - genetics
Antigenic Variation - immunology
Antigenicity
Asia
Avian flu
Birds
Birds - virology
Chickens
Chickens - immunology
Chickens - virology
Circulation
Drift
Ducks - immunology
Ducks - virology
Epidemics
H5N8
hemagglutinin
Hemagglutinin Glycoproteins, Influenza Virus - genetics
Hemagglutinin Glycoproteins, Influenza Virus - immunology
Hemagglutinins
highly pathogenic avian influenza virus
Influenza
Influenza A Virus, H5N1 Subtype - classification
Influenza A Virus, H5N1 Subtype - genetics
Influenza A Virus, H5N1 Subtype - immunology
Influenza A Virus, H5N8 Subtype - classification
Influenza A Virus, H5N8 Subtype - genetics
Influenza A Virus, H5N8 Subtype - immunology
Influenza in Birds - virology
Neutralizing
Pandemics
Polyclonal antibodies
Poultry
Poultry Diseases - immunology
Poultry Diseases - virology
Vaccines
Viruses
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Summary:ABSTRACT H5 highly pathogenic avian influenza viruses (HPAIV) have spread in both poultry and wild birds since late 2003. Continued circulation of HPAIV in poultry in several regions of the world has led to antigenic drift. In the present study, we analyzed the antigenic properties of H5 HPAIV isolated in Asia using four neutralizing mAbs recognizing hemagglutinin, which were established using A/chicken/Kumamoto/1‐7/2014 (H5N8), belonging to clade 2.3.4.4 and also using polyclonal antibodies. Viruses of clades 1.1, 2.3.2.1, 2.3.4, and 2.3.4.4 had different reactivity patterns to the panel of mAbs, thereby indicating that the antigenicity of the viruses of clade 2.3.4.4 were similar but differed from the other clades. In particular, the antigenicity of the viruses of clade 2.3.4.4 differed from those of the viruses of clades 2.3.4 and 2.3.2.1, which suggests that the recent H5 HPAIV have further evolved antigenically divergent. In addition, reactivity of antiserum suggests that the antigenicity of viruses of clade 2.3.4.4 differed slightly among groups A, B, and C. Vaccines are still used in poultry in endemic countries, so the antigenicity of H5 HPAIV should be monitored continually to facilitate control of avian influenza. The panel of mAbs established in the present study will be useful for detecting antigenic drift in the H5 viruses that emerge from the current strains.
ISSN:0385-5600
1348-0421
DOI:10.1111/1348-0421.12478