Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea

A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648 bp encodes a precursor protein of 216 amino acids, which contains a prepropeptid...

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Bibliographic Details
Published in:Toxicon (Oxford) 2002-11, Vol.40 (11), p.1563-1569
Main Authors: Jiang, Xiao-Yu, Yang, Wen-li, Chen, Hui-Ping, Tu, Hong-Bin, Wu, Wen-Yan, Wei, Jian-Wen, Wang, Juan, Liu, Wen-Hua, Xu, An-Long
Format: Article
Language:English
Subjects:
Acidic cytolysin
Amino Acid Sequence
Animal poisons toxicology. Antivenoms
Animals
Base Sequence
Biological and medical sciences
cDNA
Cloning
Cloning, Molecular
Cnidarian Venoms - genetics
Cnidarian Venoms - toxicity
cytolysins
DNA Primers
DNA, Complementary - chemistry
Hemolysin Proteins - genetics
Hemolysin Proteins - toxicity
Hemolysis - drug effects
Humans
Marine
Medical sciences
Molecular Sequence Data
Reverse Transcriptase Polymerase Chain Reaction
Sagartia rosea
Sea anemone
Sea Anemones
Src I
Src I gene
Toxicology
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Summary:A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648 bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6 kDa and a p I value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75% amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15% α-helix, 45% β-sheet, and 40% random coil. The characteristic amphiphilic α-helix of cytolysin is located at the N-terminus of the processed Src I.
ISSN:0041-0101
1879-3150
DOI:10.1016/S0041-0101(02)00173-3