The ferredoxin-dependent glutamate synthase (OsFd-GOGAT) participates in leaf senescence and the nitrogen remobilization in rice

Ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1) plays major roles in photorespiration and primary nitrogen assimilation. However, due to no mutant or knockdown lines of OsFd-GOGAT have been reported in rice ( Oryza sativa L.), the contribution of OsFd-GOGAT to rice foliar nitrogen met...

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Bibliographic Details
Published in:Molecular genetics and genomics : MGG 2017-04, Vol.292 (2), p.385-395
Main Authors: Zeng, Dong-Dong, Qin, Ran, Li, Mei, Alamin, Md, Jin, Xiao-Li, Liu, Yu, Shi, Chun-Hai
Format: Article
Language:English
Subjects:
Amino Acid Oxidoreductases - genetics
Amino Acid Oxidoreductases - metabolism
Animal Genetics and Genomics
Arabidopsis - genetics
Biochemistry
Biomedical and Life Sciences
Chlorophyll
Chromosome Mapping
Cloning, Molecular
Enzymes
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Genomics
Human Genetics
Leaves
Life Sciences
Light
Metabolism
Microbial Genetics and Genomics
Mutation
Nitrogen
Nitrogen - metabolism
Original Article
Oryza - enzymology
Oryza - genetics
Oryza sativa
Phenotype
Plant Genetics and Genomics
Plant Leaves - enzymology
Plant Leaves - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Roots - genetics
Reactive Oxygen Species - metabolism
Rice
Seeds - metabolism
Senescence
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Summary:Ferredoxin-dependent glutamate synthase (Fd-GOGAT, EC 1.4.7.1) plays major roles in photorespiration and primary nitrogen assimilation. However, due to no mutant or knockdown lines of OsFd-GOGAT have been reported in rice ( Oryza sativa L.), the contribution of OsFd-GOGAT to rice foliar nitrogen metabolism remains little up-to-date. Here, we isolated a rice premature leaf senescence mutant named gogat1 , which was reduced in 67% of the total GOGAT enzyme activity in leaves. The gogat1 mutant exhibited chlorosis under natural condition, while showed less extent premature leaf senescence under low light treatment. The gogat1 locus was mapped to a 54.1 kb region on chromosome 7, and the sequencing of OsFd-GOGAT showed one substitution (A to T) at the 3017th nucleotide of the open reading frame, leading to the amino-acid substitution of leucine changed to histidine. The gogat1 mutant showed reduced seed setting rate, while the grain protein content in gogat1 mutant was significantly higher than that in wild type. Meanwhile, during the grain-filling stage, total amino acids in the up three leaves and the upmost internode were increased dramatically. The results in this study suggested that OsFd-GOGAT might participate in nitrogen remobilization during leaf senescence, which provides a potential way to improve nitrogen use efficiency in rice.
ISSN:1617-4615
1617-4623
DOI:10.1007/s00438-016-1275-z