Bioactivities of nostocine a produced by a freshwater cyanobacterium Nostoc spongiaeforme TISTR 8169

A freshwater cyanobacterium, Nostoc spongiaeforme TISTR 8169, synthesizes and releases a violet pigment, nostocine A, into medium. We examined the bioactivity of nostocine A to several model organisms breeding with N. spongiaeforme in the natural environment. To microalgae, nostocine A exhibited gro...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2003, Vol.95 (5), p.512-517
Main Authors: Hirata, Kazumasa, Yoshitomi, Sayaka, Dwi, Susilangsih, Iwabe, Osamu, Mahakhant, Aparat, Polchai, Jirapatch, Miyamoto, Kazuhisa
Format: Article
Language:English
Subjects:
allelochemical
ALLELOCHEMICALS
ALLELOPATHY
bioactivity
cyanobacterium
FRESHWATER ECOLOGY
GROWTH INHIBITORS
NOSTOC
Nostoc spongiaeforme
nostocine A
PIGMENTS
toxin
TOXINS
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Summary:A freshwater cyanobacterium, Nostoc spongiaeforme TISTR 8169, synthesizes and releases a violet pigment, nostocine A, into medium. We examined the bioactivity of nostocine A to several model organisms breeding with N. spongiaeforme in the natural environment. To microalgae, nostocine A exhibited growth inhibitory activity comparable to paraquat, and the activity tended to be stronger to green algae than to cyanobacteria. Nostocine A also exhibited strong inhibitory activity to the root elongation of barnyard grass, strong antifeedant activity to cotton ballworm, and acute toxicity to mice resulting in its classification as a dangerous poison. The results suggest that nostocine A may act as a toxin or an allelochemical to breeding organisms in nature. In a laboratory culture of N. spongiaeforme, the production of nostocine A was enhanced at higher temperature, 30°C, and more intense light, 30 W/m2, than the basal conditions, 25°C and 10 W/m2. Cultivation of cells with H202 at 1 or 2 mM also enhanced the production of nostocine A, indicating that nostocine A may be synthesized and released when the cells are exposed to oxidative stress, possibly occurring at higher temperature and more intense light. LC-MS and electron spin resonance analyses revealed that nostocine A, reduced previously by NaBH4, immediately recovered to its original form upon exposure to air and the generation of superoxide radical anions occurred at this re-oxidation step. These results suggest that the adverse effects of nostocine A on various organisms may be related to the function of nostocine A in generating toxic reactive oxygen species, which occurs in the cells of target organisms.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(03)80053-1