Magnetic chitosan nanocomposite for hyperthermia therapy application: Preparation, characterization and in vitro experiments

Nanocrystals of magnetite (Fe3O4) were prepared by alkaline precipitation. The precursor used for synthesis was ferrous chloride only and the reaction was carried out in absence of any oxidant. The synthesized pure phase magnetic nanoparticles (MNPs) were coated with a biocompatible polymer, chitosa...

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Bibliographic Details
Published in:Applied surface science 2014, Vol.288, p.149-157
Main Authors: SHETE, P. B, PATIL, R. M, THORAT, N. D, PRASAD, A, NINGTHOUJAM, R. S, GHOSH, S. J, PAWAR, S. H
Format: Article
Language:English
Subjects:
Biomolecules
Chitosan
Chlorides
Coating
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Condensed matter: structure, mechanical and thermal properties
Cross-disciplinary physics: materials science
rheology
Exact sciences and technology
Hyperthermia
Nanoparticles
Physics
Synthesis (chemistry)
Therapy
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Summary:Nanocrystals of magnetite (Fe3O4) were prepared by alkaline precipitation. The precursor used for synthesis was ferrous chloride only and the reaction was carried out in absence of any oxidant. The synthesized pure phase magnetic nanoparticles (MNPs) were coated with a biocompatible polymer, chitosan (CS). FTIR and TGA confirm coating of CS on MNPs. Both bare and coated MNPs (Fe3O4 and CS-Fe3O4) show particle size 21.8 plus or minus 5.3 and 15.1 plus or minus 5.0nm respectively. The magnetization values of both the MNPs are 51.68 and 49.96emu/g at room temperature respectively. Negligible Coercivity and Remenance values at room temperature imply superparamagnetic behavior of the MNPs. The MNPs are studied for their induction heating abilities at 167.6, 251.4 and 335.2Oe (equivalent to 13.3, 20.0 and 26.7kAm-1 respectively), in order to use them in magnetic fluid hyperthermia therapy. At 335.2Oe, CS coated nanoparticles (NPs) show maximum SAR of 118.85W/g, while bare NPs show SAR of 79.32W/g. Low cytotoxic effects of both the MNPs on L929 cell line proved their suitability for in vivo applications. --NH2 group rendered by CS can further be used for conjugation of biomolecules to make them suitable candidates for biosensing and targeted drug delivery.
ISSN:0169-4332
1873-5584
DOI:10.1016/j.apsusc.2013.09.169