Lipidomics analysis of long‐chain fatty acyl‐coenzyme As in liver, brain, muscle and adipose tissue by liquid chromatography/tandem mass spectrometry

Rationale Long‐chain fatty acyl‐coenzyme As (FA‐CoAs) are important bioactive molecules, playing key roles in biosynthesis of fatty acids, membrane trafficking and signal transduction. Development of sensitive analytical methods for profiling theses lipid species in various tissues is critical to un...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2017-02, Vol.31 (4), p.344-350
Main Authors: Morin‐Rivron, Delphine, Christinat, Nicolas, Masoodi, Mojgan
Format: Article
Language:English
Subjects:
Acyl Coenzyme A - analysis
Adipose Tissue - enzymology
Adipose tissues
Animals
Brain
Brain - enzymology
Chromatography, Liquid - methods
Extraction
Liquid chromatography
Liver
Liver - enzymology
Male
Mass spectrometry
Muscles
Muscles - enzymology
Organ Specificity
Rats
Rats, Sprague-Dawley
Screening
Tandem Mass Spectrometry - methods
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Summary:Rationale Long‐chain fatty acyl‐coenzyme As (FA‐CoAs) are important bioactive molecules, playing key roles in biosynthesis of fatty acids, membrane trafficking and signal transduction. Development of sensitive analytical methods for profiling theses lipid species in various tissues is critical to understand their biological activity. A high‐pressure liquid chromatography/tandem mass spectrometry method has been developed for the quantitative analysis and screening of long‐chain FACoAs in liver, brain, muscle and adipose tissue. Methods The sample preparation method consists of tissue homogenization, extraction with organic solvent and reconstitution in an ammonium hydroxide buffer. Extracts are separated by liquid chromatography (LC) on a reversed‐phase column and detected by electrospray ionization tandem mass spectrometry (ESI‐MS/MS) in positive mode. An additional neutral loss scan allows for untargeted FA‐CoAs screening. Results Extraction was optimized for low sample load (10 mg) of four tissue types (liver, brain, muscle and adipose tissue) with recoveries between 60–140% depending on the analyte and tissue type. Targeted quantification was validated for ten FA‐CoAs in the range 0.1–500 ng/mL with accuracies between 85–120%. Conclusions We have developed and validated a LC/MS/MS method for the quantifications and screening of long‐chain FA‐CoAs in four different types of mammalian tissue. The extraction method is straightforward and long‐chain FA‐CoA species can be quantified using only minimum amount of tissue. Copyright © 2016 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.7796