Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men

We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal wei...

Full description

Saved in:

Bibliographic Details
Published in:	The Journal of nutritional biochemistry 2017-05, Vol.43, p.156-165
Main Authors:	Gille, Doreen, Zangger, Nadine, Soneson, Charlotte, Bütikofer, Ueli, Delorenzi, Mauro, Schwander, Flurina, Kopf-Bolanz, Katrin A., Chollet, Magali, Walther, Barbara, Laederach, Kurt, Vergères, Guy
Format:	Article
Language:	eng
Subjects:	Adult Diet, High-Fat Dose–Response Energy Intake - genetics Gene expression Gene Expression Regulation High-fat meal Humans Insulin Lipids - blood Male Middle Aged Multivariate Analysis Obesity - blood Obesity - genetics Oxidative phosphorylation Postprandial Period
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to investigate differentially expressed genes in both study groups. We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response indicative of a loss of metabolic flexibility. The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may represent a sensitive way to evaluate the qualitative impact of food on human health.
ISSN:	0955-2863 1873-4847