miRNA Expression Change in Dorsal Root Ganglia After Peripheral Nerve Injury

The role of microRNAs (miRNAs) in the regulation of nerve injury-induced neuropathic pain is unclear. The aims of this study were to assess and compare miRNA expression profiles in dorsal root ganglia (DRG) following three different kinds of peripheral nerve injury, including spinal nerve ligation (...

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Bibliographic Details
Published in:Journal of molecular neuroscience 2017-02, Vol.61 (2), p.169-177
Main Authors: Chang, Hsueh-Ling, Wang, Hung-Chen, Chunag, Yi-Ta, Chou, Chao-Wen, Lin, I-Ling, Lai, Chung-Sheng, Chang, Lin-Li, Cheng, Kuang-I
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Ganglia, Spinal - metabolism
Hospitals
Hybridization
Laboratory animals
Male
Medical schools
Medicine
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Neurochemistry
Neurology
Neurosciences
Pain
Peripheral Nerve Injuries - genetics
Peripheral Nerve Injuries - metabolism
Proteomics
Rats
Rats, Sprague-Dawley
Skin
Surgery
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Summary:The role of microRNAs (miRNAs) in the regulation of nerve injury-induced neuropathic pain is unclear. The aims of this study were to assess and compare miRNA expression profiles in dorsal root ganglia (DRG) following three different kinds of peripheral nerve injury, including spinal nerve ligation (SNL), dorsal root transection (DRT), and ventral root transection (VRT), in Sprague–Dawley rats. Responses to thermal and mechanical stimuli were measured preoperatively and on postoperative days (PODs) 1, 4, and 7. A miRNA microarray analysis was used to detect the miRNA expression profiles in injured L5 DRG from SNL, DRT, and VRT on POD 7. Validation of miRNA expression was performed by qPCR and in situ hybridization. Rats receiving SNL displayed significantly higher mechanical hypersensitivity, but those receiving DRT developed higher thermal hypersensitivity. The number of miRNAs that were significantly upregulated in L5 DRG was 49 (7.2%), 25 (3.7%), and 146 (21.5%) following SNL, DRT, and VRT, respectively. On the other hand, 35 (5.1%) miRNAs were significantly downregulated in the SNL group, 21 (3.1%) miRNAs in the DRT group, and 41 (6.0%) miRNAs in the VRT group. Of the four miRNAs that were mutually aberrant in all three models, two were significantly upregulated (twofold), miR-21 and miR-31, and two were significantly downregulated, miR-668 and miR-672. Using in situ hybridization, miRNA-21, miRNA-31, miRNA-668, and miRNA-672 were found to localize to neurons in the DRG. Collectively, the mutual abnormal miRNA expression of miR-21, miR-31, miR-668, and miR-677 implied that these miRNAs may be therapeutic targets for alleviating multiple forms of neuropathic pain.
ISSN:0895-8696
1559-1166
DOI:10.1007/s12031-016-0876-7