Characterization of virulence factors and phylogenetic group determination of Escherichia coli isolated from diarrheic and non-diarrheic calves from Brazil

This study aimed to detect virulence factors, pathovars, and phylogenetic groups of Escherichia coli strains obtained from feces of calves with and without diarrhea up to 70 days old and to determine the association between occurrence of diarrhea, phylogenetic groups, and pathovars. Phylo-typing ana...

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Bibliographic Details
Published in:Folia microbiologica 2017-03, Vol.62 (2), p.139-144
Main Authors: Coura, Fernanda Morcatti, de Araújo Diniz, Soraia, Mussi, Jamili Maria Suhet, Silva, Marcos Xavier, Lage, Andrey Pereira, Heinemann, Marcos Bryan
Format: Article
Language:English
Subjects:
Animals
Applied Microbiology
Bacterial Typing Techniques
Biomedical and Life Sciences
Brazil
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - microbiology
Cattle Diseases - pathology
Diarrhea
Diarrhea - diagnosis
Diarrhea - microbiology
Diarrhea - pathology
Diarrhea - veterinary
Digestive system
DNA, Bacterial - genetics
E coli
Enteropathogenic Escherichia coli - classification
Enteropathogenic Escherichia coli - genetics
Enteropathogenic Escherichia coli - growth & development
Enteropathogenic Escherichia coli - pathogenicity
Enterotoxigenic Escherichia coli - classification
Enterotoxigenic Escherichia coli - genetics
Enterotoxigenic Escherichia coli - growth & development
Enterotoxigenic Escherichia coli - pathogenicity
Environmental Engineering/Biotechnology
Escherichia coli
Escherichia coli Infections - diagnosis
Escherichia coli Infections - microbiology
Escherichia coli Infections - pathology
Escherichia coli Infections - veterinary
Feces - microbiology
Gene Expression
Genotype
Immunology
Life Sciences
Male
Microbiology
Pathogens
Phylogenetics
Phylogeny
Polymerase Chain Reaction
Shiga-Toxigenic Escherichia coli - classification
Shiga-Toxigenic Escherichia coli - genetics
Shiga-Toxigenic Escherichia coli - growth & development
Shiga-Toxigenic Escherichia coli - pathogenicity
Virulence
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:This study aimed to detect virulence factors, pathovars, and phylogenetic groups of Escherichia coli strains obtained from feces of calves with and without diarrhea up to 70 days old and to determine the association between occurrence of diarrhea, phylogenetic groups, and pathovars. Phylo-typing analysis of the 336 E. coli strains isolated from calves with Clermont method showed that 21 (6.25 %) belong to phylogroup A, 228 (67.85 %) to phylogroup B1, 2 (0.6 %) to phylogroup B2, 5 (1.49 %) to phylogroup C, 57 (16.96 %) to phylogroup E, and 3 (0.9 %) to phylogroup F. Phylogroup D was not identified and 20 strains (5.95 %) were assigned as “unknown.” The distribution of phylogenetic groups among pathovars showed that NTEC belong to phylogroups B1 (17) and C (4); EPEC to phylogroups B1 (6) and E (8); STEC to phylogroups A (5), B1 (56), B2 (2), C (1), and E (15); EHEC to phylogroups B1 (95) and E (5); and ETEC to phylogroups A (3), B1 (7), and E (10). The EAST-1 strains were phylogroups A (13), B1 (47), E (19), and F (3); E. coli strains of “unknown” phylogroups belonged to pathovars EPEC (1), EHEC (2), STEC (7), and EAST-1 strains (6). ETEC was associated with diarrhea ( P  = 0.002). Our study did not find association between the phylogenetic background and occurrence of diarrhea ( P  = 0.164) but did find some relationship in phylogenetic group and pathovar. The study showed that EHEC and STEC are classified as phylogroup B1, EAST-1 phylogroup A, ETEC, and EPEC phylogroup E.
ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-016-0480-9