Magnetite Nanoparticles for Stem Cell Labeling with High Efficiency and Long-Term in Vivo Tracking

Superparamagnetic iron oxide nanoparticles (SPIO-PAA), ultrasmall iron oxide nanoparticles (USPIO-PAA), and glucosamine-modified iron oxide nanoparticles (USPIO-PAA-GlcN) were studied as mesenchymal stem cell (MSCs) labels for cell tracking applications by magnetic resonance imaging (MRI). Pronounce...

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Bibliographic Details
Published in:Bioconjugate chemistry 2017-02, Vol.28 (2), p.362-370
Main Authors: Guldris, Noelia, Argibay, Bárbara, Gallo, Juan, Iglesias-Rey, Ramón, Carbó-Argibay, Enrique, Kolen’ko, Yury V, Campos, Francisco, Sobrino, Tomás, Salonen, Laura M, Bañobre-López, Manuel, Castillo, José, Rivas, José
Format: Article
Language:English
Subjects:
Animals
Biological Transport
Cell Survival - drug effects
Cell Tracking - methods
Chemical compounds
Efficiency
Iron
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - toxicity
Male
Materials Testing
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Minerals
Nanoparticles
Particle Size
Rats
Staining and Labeling
Stem cells
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Summary:Superparamagnetic iron oxide nanoparticles (SPIO-PAA), ultrasmall iron oxide nanoparticles (USPIO-PAA), and glucosamine-modified iron oxide nanoparticles (USPIO-PAA-GlcN) were studied as mesenchymal stem cell (MSCs) labels for cell tracking applications by magnetic resonance imaging (MRI). Pronounced differences were found in the labeling performance of the three samples in terms of cellular dose and labeling efficiency. In combination with polylysine, SPIO-PAA showed nonhomogeneous cell internalization, while for USPIO-PAA no uptake was found. On the contrary, USPIO-PAA-GlcN featured high cellular uptake and biocompatibility, and sensitive detection in both in vitro and in vivo experiments was found by MRI, showing that glucosamine functionalization can be an efficient strategy to increase cell uptake of ultrasmall iron oxide nanoparticles by MSCs.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.6b00522