Determination of eurycomanone in rat plasma using hydrophilic interaction liquid chromatography–tandem mass spectrometry for pharmacokinetic study

In this study, a rapid, sensitive, and reliable hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC‐MS/MS) method for the determination of eurycomanone in rat plasma was developed and validated. Plasma samples were pretreated with a protein precipitation method and quercitr...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical chromatography 2017-04, Vol.31 (4), p.np-n/a
Main Authors: Rehman, Shaheed Ur, Choi, Min Sun, Han, Young Min, Kim, In Sook, Kim, Seung Hyun, Piao, Xiang‐Lan, Yoo, Hye Hyun
Format: Article
Language:English
Subjects:
Animals
Area Under Curve
Calibration
Chromatography, Liquid - methods
Eurycoma - chemistry
Eurycoma longifolia
eurycomanone
HILIC‐MS/MS
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Male
pharmacokinetics
Plant Extracts - administration & dosage
Plant Extracts - blood
Plant Extracts - pharmacokinetics
plasma
Quassins - blood
Quassins - pharmacokinetics
Rats, Sprague-Dawley
Reproducibility of Results
Tandem Mass Spectrometry - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, a rapid, sensitive, and reliable hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC‐MS/MS) method for the determination of eurycomanone in rat plasma was developed and validated. Plasma samples were pretreated with a protein precipitation method and quercitrin was used as an internal standard (IS). A HILIC silica column (2.1 × 100 mm, 3 μm) was used for hydrophilic‐based chromatographic separation, using the mobile phase of 0.1% formic acid with acetonitrile in gradient elution at a flow rate of 0.25 mL/min. Precursor–product ion pairs for multiple‐reaction monitoring were m/z 409.1 → 391.0 for eurycomanone and m/z 449.1 → 303.0 for IS. The linear range was 2–120 ng/mL. The intra‐ and inter‐day accuracies were between 95.5 and 103.4% with a precision of
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3831