Lipophilization of Hydroxytyrosol-Enriched Fractions from Olea europaea L. Byproducts and Evaluation of the in Vitro Effects on a Model of Colorectal Cancer Cells

A hydroxytyrosol (HTyr)-enriched fraction containing HTyr 6% w/w, derived from Olea europaea L. byproducts and obtained using an environmentally and economically sustainable technology, was lipophilized under green chemistry conditions. The effects of three fractions containing hydroxytyrosyl butano...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2017-08, Vol.65 (31), p.6506-6512
Main Authors: Bernini, Roberta, Carastro, Isabella, Palmini, Gaia, Tanini, Annalisa, Zonefrati, Roberto, Pinelli, Patrizia, Brandi, Maria Luisa, Romani, Annalisa
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Colorectal Neoplasms - physiopathology
Estrogen Receptor beta - genetics
Estrogen Receptor beta - metabolism
Humans
Molecular Structure
Olea - chemistry
Phenylethyl Alcohol - analogs & derivatives
Phenylethyl Alcohol - chemistry
Phenylethyl Alcohol - pharmacology
Plant Extracts - chemistry
Plant Extracts - pharmacology
Waste Products - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A hydroxytyrosol (HTyr)-enriched fraction containing HTyr 6% w/w, derived from Olea europaea L. byproducts and obtained using an environmentally and economically sustainable technology, was lipophilized under green chemistry conditions. The effects of three fractions containing hydroxytyrosyl butanoate, octanoate, and oleate, named, respectively, lipophilic fractions 5, 6, and 7, and unreacted HTyr on the human colon cancer cell line HCT8-β8 engineered to overexpress estrogen receptor β (ERβ) were evaluated and compared to those of pure HTyr. The experimental data demonstrated that HTyr and all fractions showed an antiproliferative effect, as had been observed by the evaluation of the cellular doubling time under these different conditions (mean control, 32 ± 4 h; HTyr 1, 65 ± 9 h; fraction 5, 64 ± 11 h; fraction 6, 62 ± 14 h; fraction 7, 133 ± 30 h). As evidenced, fraction 7 containing hydroxytyrosyl oleate showed the highest activity. These results were related to the link with ER-β, which was assessed through simultaneous treatment with an inhibitor of ERβ.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.6b05457