Serum biomarkers of oxidative stress in dogs with idiopathic inflammatory bowel disease

•Decreased total antioxidant capacity, measured by CUPRAC and TEAC assays, was demonstrated in sera from dogs with IBD.•Serum thiol and PON1 activity was significantly decreased in sera from dogs with IBD compared with healthy dogs.•Oxidative damage in dogs with IBD was demonstrated by increased ser...

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Bibliographic Details
Published in:The veterinary journal (1997) 2017-03, Vol.221, p.56-61
Main Authors: Rubio, C.P., Martínez-Subiela, S., Hernández-Ruiz, J., Tvarijonaviciute, A., Cerón, J.J., Allenspach, K.
Format: Article
Language:English
Subjects:
Animals
Antioxidant
Aryldialkylphosphatase - blood
Biomarkers - blood
Case-Control Studies
Cupric
Dog Diseases - blood
Dogs
Female
Inflammatory Bowel Diseases - blood
Inflammatory Bowel Diseases - veterinary
Male
Oxidative Stress
Oxygen Radical Absorbance Capacity
PON1
Reactive oxygen species
Reactive Oxygen Species - blood
Retrospective Studies
Sulfhydryl Compounds - blood
Thiol
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Summary:•Decreased total antioxidant capacity, measured by CUPRAC and TEAC assays, was demonstrated in sera from dogs with IBD.•Serum thiol and PON1 activity was significantly decreased in sera from dogs with IBD compared with healthy dogs.•Oxidative damage in dogs with IBD was demonstrated by increased serum FOX, ROS and TBARS. The objective of this work was to study and compare a panel of various serum biomarkers evaluating both the antioxidant response and oxidative damage in dogs with idiopathic inflammatory bowel disease (IBD). Eighteen dogs with IBD and 20 healthy dogs were enrolled in the study. Trolox equivalent antioxidant capacity (TEAC), cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of the plasma (FRAP), total thiol concentrations, and paraoxonase 1 (PON1) activity were evaluated in serum to determine antioxidant response. To evaluate oxidative status, ferrous oxidation-xylenol orange (FOX), thiobarbituric acid reactive substances (TBARS) and reactive oxygen species production (ROS) concentrations in serum were determined. Mean concentrations of all antioxidant biomarkers analyzed, with exception of FRAP, were significantly lower (P 
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2017.02.003