DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma

Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether in...

Full description

Saved in:
Bibliographic Details
Published in:Cancer letters 2004-06, Vol.209 (2), p.207-213
Main Authors: Hewitt, Chelsee, Wilson, Peter, McGlinn, Edwina, MacFarlane, Gary, Papageorgiou, Anne, Woodwards, Robert T.M, Sloan, Philip, Gollin, Susanne M, Paterson, Ian, Parkinson, Kenneth K, Read, Andrew P, Thakker, Nalin
Format: Article
Language:English
Subjects:
Atmospheric pressure
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Chromosome Deletion
Chromosomes, Human, Pair 8 - genetics
Demonstrations & protests
DLC1
Federal court decisions
GTPase-Activating Proteins
Head and Neck Neoplasms - genetics
Head and Neck Neoplasms - pathology
Head and neck squamous cell carcinoma
Humans
Liver cancer
Loss of Heterozygosity
Memorials & monuments
Microsatellite Repeats
Mutation
Polymorphism, Genetic
Stone
Tumor Cells, Cultured
Tumor Suppressor Proteins - genetics
Tumor Suppressor Proteins - metabolism
Tumour suppressor gene
Unexplained phenomena
Witchcraft
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened tumour cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2003.12.018