Starch materials as biocompatible supports and procedure for fast separation of macrophages

•Gelatinized Starch and Acetate Starch are good supports for macrophage adhesion.•Ionic Carboxylic and Amino starch derivatives are less compatible with macrophages.•Macrophages are released by enzymatic hydrolysis of starch films with alpha-amylase.•Macrophage viability is higher with hydrolysed fi...

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Bibliographic Details
Published in:Carbohydrate polymers 2017-05, Vol.163, p.108-117
Main Authors: Sakeer, Khalil, Scorza, Tatiana, Romero, Hugo, Ispas-Szabo, Pompilia, Mateescu, Mircea Alexandru
Format: Article
Language:English
Subjects:
Acetate starch
Alpha-amylase
alpha-Amylases
Animals
Biocompatible Materials
Cell Separation - methods
Gelatinized starch
Hydrolysis
Macrophage separation
Macrophages - cytology
Mice
RAW 264.7 Cells
Starch - chemistry
Starch derivatives
Tumor necrosis factor (TNF-α)
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Summary:•Gelatinized Starch and Acetate Starch are good supports for macrophage adhesion.•Ionic Carboxylic and Amino starch derivatives are less compatible with macrophages.•Macrophages are released by enzymatic hydrolysis of starch films with alpha-amylase.•Macrophage viability is higher with hydrolysed films than with other current methods.•Macrophage responses to lipopolysaccharide are not affected by the proposed procedure. Different starch derivatives were evaluated as supports for attachment and recovery of macrophages (RAW 264.7 line). Gelatinized starch (G-St), acetate starch (Ac-St), carboxymethyl starch and aminoethyl starch were synthesized and characterized by FTIR, 1H NMR, SEM and static water contact angle. These polymers are filmogenic and may coat well the holder devices used for macrophage adhesion. They also present a susceptibility to mild hydrolysis with alpha-amylase, liberating the adhered macrophages. Cell counts, percentage of dead cells and level of tumor necrosis factor (TNF-α) were used to evaluate the possible interaction between macrophages and starch films. The high percentage of cell adhesion (90–95% on G-St and on Ac-St) associated with enzymatic detachment of macrophages from film-coated inserts, resulted in higher viabilities compared with those obtained with cells detached by current methods scrapping or vortex. This novel method allows a fast macrophage separation, with excellent yields and high viability of recovered cells.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2017.01.053