Reliability of automated synovial fluid cell counting with Mindray BC‐6800 body fluid mode

Introduction The enumeration and differentiation of nuclear elements in synovial fluid is a cornerstone for diagnosis and follow‐up of many orthopedic and rheumatologic diseases. In this study, we evaluated the analytical performance of Mindray BC‐6800 BF mode (BC‐6800‐BF) for synovial fluid analysi...

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Published in:International journal of laboratory hematology 2017-06, Vol.39 (3), p.337-346
Main Authors: Buoro, S., Seghezzi, M., Manenti, B., Mecca, T., Candiago, E., Vidali, M., Ottomano, C., Castelli, C.C., Ferrari, R., Zappalà, G., Lippi, G.
Format: Article
Language:English
Subjects:
BC‐6800
body fluid
Enumeration
Female
Flow Cytometry - instrumentation
Flow Cytometry - methods
Fluids
Humans
Hyaluronoglucosaminidase - chemistry
Leukocytes
Leukocytes - metabolism
Leukocytes - pathology
light microscopy
Maintenance management
Male
Microscopy
Middle Aged
Mindray
Rheumatic Diseases - metabolism
Rheumatic Diseases - pathology
Synovial fluid
Synovial Fluid - metabolism
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Summary:Introduction The enumeration and differentiation of nuclear elements in synovial fluid is a cornerstone for diagnosis and follow‐up of many orthopedic and rheumatologic diseases. In this study, we evaluated the analytical performance of Mindray BC‐6800 BF mode (BC‐6800‐BF) for synovial fluid analysis. Methods Overall, 78 synovial fluids were collected and analyzed with both BC‐6800‐BF and light microscopy. The study also entailed the assessment of limit of blank (LoB), limit of detection (LoD), limit of quantification (LoQ), carryover and linearity. Results The LoB for the parameters total cells and white blood cells was 6 × 106 cells/L, and the LoD and LoQ were instead 15 and 16 × 106 cells/L, respectively. Linearity was excellent and carryover was negligible. The agreement between BC‐6800‐BF and light microscopy was satisfactory for all samples pretreated with hyaluronidase, displaying a bias between −5.9% and 8.2%. Conclusions The use of BC‐6800‐BF for synovial fluid analysis enables rapid and accurate assessment, especially for total cell and polymorphonuclear counts. The use of BC‐6800‐BF may therefore allow the replacement of optical analysis, especially in samples pretreated with hyaluronidase, thus allowing its routine use for the screening of synovial specimens.
ISSN:1751-5521
1751-553X
DOI:10.1111/ijlh.12632