Construction of a bacterial biosensor for styrene

A new bacterial biosensor for styrene has been developed and characterized. A translational fusion of the lacZ gene to the sty promoter of Pseudomonas sp. strain Y2 has been inserted into miniTn 5. Transposition of the recombinant transposon to the chromosome of Pseudomonas sp. strain Y2 resulted in...

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Bibliographic Details
Published in:Journal of biotechnology 2003-05, Vol.102 (3), p.301-306
Main Authors: Alonso, Sergio, Navarro-Llorens, Juana Marı&#x0301, a, Tormo, Antonio, Perera, Julián
Format: Article
Language:English
Subjects:
Aromatic compounds
beta-Galactosidase - analysis
beta-Galactosidase - biosynthesis
Biological and medical sciences
Biosensing Techniques
Biosensor
Cell Division - drug effects
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation - physiology
Genetic Engineering - methods
Industrial Waste - analysis
Pseudomonas - classification
Pseudomonas - genetics
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Styrene
Styrenes - analysis
Styrenes - metabolism
Toluene
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Summary:A new bacterial biosensor for styrene has been developed and characterized. A translational fusion of the lacZ gene to the sty promoter of Pseudomonas sp. strain Y2 has been inserted into miniTn 5. Transposition of the recombinant transposon to the chromosome of Pseudomonas sp. strain Y2 resulted in a whole-cell biosensor able to detect and degrade styrene. In this biosensor, the endogenous StyS/StyR system detects the presence of styrene and turns on the expression of the exogenous reporter gene from the transferred construction. Other compounds such as toluene, epoxystyrene, phenylacetaldehyde and 2-phenylethanol also induced expression of β-galactosidase although quantitative differences in their effect are clearly detected. Non-inducing compounds affect differently the sensitivity to inducing compounds when present in a mixture.
ISSN:0168-1656
1873-4863
DOI:10.1016/S0168-1656(03)00048-8