Tissue-specific induction of cytochromes P450 1A1 and 1B1 by polycyclic aromatic hydrocarbons and polychlorinated biphenyls in engineered C57BL/6J mice of arylhydrocarbon receptor gene

Tissue-specific induction of mRNA of cytochrome P450 (P450 or CYP) 1A1 and 1B1 by polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) was investigated in wild and arylhydrocarbon receptor (AhR)-deficient C57BL/6J mice. Ratios of mRNA expression of CYP1A1 or CYP1B1 over β-act...

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Bibliographic Details
Published in:Toxicology and applied pharmacology 2003-02, Vol.187 (1), p.1-10
Main Authors: Shimada, Tsutomu, Sugie, Atsushi, Shindo, Masaki, Nakajima, Takae, Azuma, Emiko, Hashimoto, Masafumi, Inoue, Kiyoshi
Format: Article
Language:English
Subjects:
Actins - metabolism
Animals
Aryl Hydrocarbon Hydroxylases - biosynthesis
Arylhydrocarbon receptor
Benzo[ a]pyrene
Biological and medical sciences
Chemical and industrial products toxicology. Toxic occupational diseases
CYP1A1
CYP1B1
Cytochrome P-450 CYP1A1 - biosynthesis
Cytochrome P-450 CYP1B1
Enzyme Induction - drug effects
Female
Induction
Lung - drug effects
Lung - enzymology
Male
Medical sciences
Mice
Mice, Inbred C57BL
Microsomes, Liver - drug effects
Microsomes, Liver - enzymology
Polychlorinated Biphenyls - pharmacology
Polycyclic aromatic hydrocarbon
Polycyclic Aromatic Hydrocarbons - pharmacology
Receptors, Aryl Hydrocarbon - deficiency
Receptors, Aryl Hydrocarbon - drug effects
Receptors, Aryl Hydrocarbon - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
Toxicology
Various organic compounds
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Summary:Tissue-specific induction of mRNA of cytochrome P450 (P450 or CYP) 1A1 and 1B1 by polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) was investigated in wild and arylhydrocarbon receptor (AhR)-deficient C57BL/6J mice. Ratios of mRNA expression of CYP1A1 or CYP1B1 over β-actin were determined and used to compare levels of expression and induction of these P450s by PAHs and PCBs in various organs. CYP1A1 mRNA was detected in control mice at very low levels in liver, lung, heart, kidney, intestine, thymus, testis, uterus, ovary, and brain and was highly induced in these organs by benzo[ a]pyrene and 3,4,3′,4′-tetrachlorobiphenyl in AhR(+/+) mice. In AhR(+/+) and AhR(−/−) mice, CYP1B1 mRNA was found to be constitutively expressed at significant levels in heart (the ratio of mRNAs of CYP1B1 to β-actin was ∼0.6), kidney (∼0.8), intestine (∼0.3), testis (∼0.9), thymus (∼0.4), uterus (∼0.3), ovary (∼1.4), and brain (∼0.4), whereas it was low in liver and lung (the mRNA ratio to β-actin was
ISSN:0041-008X
1096-0333
DOI:10.1016/S0041-008X(02)00035-2