Microbial community of Pacific abalone (Haliotis discus hannai) juveniles during a disease outbreak in South China

A mass mortality of farmed juvenile abalones (Haliotis discus hannai) occurred in South China in December of 2012. Combined traditional culturing with terminal restriction fragment length polymorphism (T‐RFLP) methods, microbial communities associated with juvenile abalones were investigated under d...

Full description

Saved in:
Bibliographic Details
Published in:Aquaculture research 2017-03, Vol.48 (3), p.1080-1088
Main Authors: Shi, Liu‐yang, Liang, Shuang, Luo, Xuan, Ke, Cai‐huan, Zhao, Jing
Format: Article
Language:English
Subjects:
cultivation
disease
Haliotis
Haliotis discus hannai
juvenile abalone
Marine
microbiota
Photobacterium
Staphylococcus haemolyticus
survival condition
T‐RFLP
Vibrio
Vibrionaceae
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A mass mortality of farmed juvenile abalones (Haliotis discus hannai) occurred in South China in December of 2012. Combined traditional culturing with terminal restriction fragment length polymorphism (T‐RFLP) methods, microbial communities associated with juvenile abalones were investigated under different survival conditions, including healthy (HA) and moribund (HM) abalones in the healthy pond with low mortalities, and healthy (DA) and moribund (DM) abalones in the diseased pond with a high mortality rate. The results indicated that both HA and DM exhibited greater microbial diversity and evenness. Moreover, the number of bacterial colony forming units (CFUs) from HA and DM were higher than other samples from the same pond. HA and DA samples harboured absolutely dominant T‐RFs with 504 bp (76.2% of total T‐RFs) and 436 bp (63.2%) respectively. Nesterenkonia sandarakina, Staphylococcus haemolyticus and Agarivorans albus were the dominate isolates in HA samples, but no Vibrio genus was isolated even on vibrio‐specific medium. Reversely, in the DM samples, up to 94% of the total bacterial CFUs were composed of the family Vibrionaceae, represented by Photobacterium swingsii (44%) and Vibrio shilonii (45%). Also, Vibrio was the only cultivated genus from the HM group. Of these Vibrio species, V. atlanticus were detected in HM, DA and DM samples and could generate the T‐RF 504 bp. The microbial composition and diversity of juvenile abalones in different surviving states provided insights into the stability and dynamics of microbial communities during disease outbreak which could be helpful to predict and to control disease in the future.
ISSN:1355-557X
1365-2109
DOI:10.1111/are.12950