Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita

The full cDNA of Mi-ace-3 encoding an acetylcholinesterase (AChE) in Meloidogyne incognita was cloned and characterized. Mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. Key residues essential to AChE structure and function were conserved. The deduced Mi-ACE-3 protein...

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Bibliographic Details
Published in:Experimental parasitology 2017-05, Vol.176, p.75-81
Main Authors: Cui, Ruqiang, Zhang, Lei, Chen, Yuyan, Huang, Wenkun, Fan, Chengming, Wu, Qingsong, Peng, Deliang, da Silva, Washington, Sun, Xiaotang
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Acetylcholinesterase - chemistry
Acetylcholinesterase - genetics
Acetylcholinesterase - metabolism
Amino Acid Sequence
Animals
Cloning, Molecular
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA, Helminth - chemistry
DNA, Helminth - genetics
Evolution, Molecular
Expression
Gene Expression Regulation, Enzymologic
Gene Knockdown Techniques
Meloidogyne incognita
Nicotiana - enzymology
Nicotiana - genetics
Nicotiana - parasitology
Nucleic Acid Amplification Techniques
Open Reading Frames
Phylogenetic analysis
Phylogeny
Plant Roots - parasitology
Plant Tumors - genetics
Plant Tumors - parasitology
RNA Interference - physiology
RNAi
Solanum lycopersicum - parasitology
Tylenchoidea - classification
Tylenchoidea - enzymology
Tylenchoidea - genetics
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Summary:The full cDNA of Mi-ace-3 encoding an acetylcholinesterase (AChE) in Meloidogyne incognita was cloned and characterized. Mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. Key residues essential to AChE structure and function were conserved. The deduced Mi-ACE-3 protein sequence had 72% amino acid similarity with that of Ditylenchus destructor Dd-AChE-3. Phylogenetic analyses using 41 AChEs from 24 species showed that Mi-ACE-3 formed a cluster with 4 other nematode AChEs. Our results revealed that the Mi-ace-3 cloned in this study, which is orthologous to Caenorhabditis elegans AChE, belongs to the nematode ACE-3/4 subgroup. There was a significant reduction in the number of galls in transgenic tobacco roots when Mi-ace-1, Mi-ace-2, and Mi-ace-3 were knocked down simultaneously, whereas little or no effect were observed when only one or two of these genes were knocked down. This is an indication that the functions of these three genes are redundant. [Display omitted] •High expression level of Mi-ace-1 persisted in M. incognita female stage.•High expression level of Mi-ace-2 and Mi-ace-3 persisted in the par-J4 stage of M. incognita.•In planta RNAi silencing of Mi-aces reduced the parasitism ability of M. incognita.•The functions of these three ace genes of M. incognita are redundant.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2017.01.008